Interaction of the Tn7-encoded transposition protein TnsB with the ends of the transposon

Lidia K. Arciszewska, Nancy L. Craig

Research output: Contribution to journalArticlepeer-review

37 Scopus citations


We have used several high resolution methods to examine the interaction of TnsB, a transposition protein encoded by the bacterial transposon Tn7, with its binding sites at the ends of the transposon. These binding sites lie within the DNA segments that are directly involved in transposition. We show that the binding of TnsB to DNA can promote DNA bending, suggesting that the interaction of TnsB with the ends may result in formation of a highly organized protein-DNA complex. We also Identify likely positions of close contact between TnsB and its binding sites. Analysis of the interaction of TnsB with Intact Tn7 ends reveals TnsB occupies its binding sites in a particular order, the sites immediately adjacent to the transposon termini being occupied only after other inner sites are bound. Such ordered occupancy suggests that the various binding sites have differing apparent affinities for TnsB.

Original languageEnglish (US)
Pages (from-to)5021-5029
Number of pages9
JournalNucleic acids research
Issue number18
StatePublished - Sep 25 1991
Externally publishedYes

ASJC Scopus subject areas

  • Genetics


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