Interaction of the N-ethylmaleimide-sensitive factor with AMPA receptors

Insuk Song, Sunjeev Kamboj, Jun Xia, Hualing Dong, Dezhi Liao, Richard L. Huganir

Research output: Contribution to journalArticlepeer-review

342 Scopus citations


Glutamate receptors mediate the majority of rapid excitatory synaptic transmission in the central nervous system (CNS) and play important roles in synaptic plasticity and neuronal development. Recently, protein-protein interactions with the C-terminal domain of glutamate receptor subunits have been shown to be involved in the modulation of receptor function and clustering at excitatory synapses. In this paper, we have found that the N- ethylmaleimide-sensitive factor (NSF), a protein involved in membrane fusion events, specifically interacts with the C terminus of the GluR2 and GluR4c subunits of AMPA receptors in vitro and in vivo. Moreover, intracellular perfusion of neurons with a synthetic peptide that competes with the interaction of NSF and AMPA receptor subunits rapidly decreases the amplitude of miniature excitatory postsynaptic currents (mEPSCs), suggesting that NSF regulates AMPA receptor function.

Original languageEnglish (US)
Pages (from-to)393-400
Number of pages8
Issue number2
StatePublished - Aug 1998

ASJC Scopus subject areas

  • Neuroscience(all)


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