Interaction of psoralen-derivatlzed oligodeoxyribonucleoside methyiphosphonates with synthetic DNA containing a promoter for 11 RNA polymerase

Bok L. Lee, Kathleen Blake, Paul S. Miller

Research output: Contribution to journalArticle

Abstract

The interaction of 4′-N(2-aminoethyl)aminomethyl-4, 5′,8- trimethylpsoralen-modif led 01 igonucleoside methyiphosphonates with synthetic ds-DNA containing a T7 RNA polymerase promoter was studied. The oligomers effectively crosslin.ked with either coding or noncoding as-DNA when irradiated at 365 rim, but not with ds-DNA. The extent of the crosslinking reaction, which was complete within 16 mm: (a) reached its maximum at an oligomer concentration of 3 μM; (b) remained constant below the Tin of the duplex and then rapidly decreased; and (c) appeared to depend upon the sequence surrounding the psoralen crosslinking site. An oligomer crosslinked to the template strand inhibited tran scription by T7 RNA polymerase whereas an oligoiner crosslinked to the non-template strand had only a small inhibitory effect. Oligomers did not crosslink to ds-DNA undergoing transcription nor did they inhibit the transcription reaction.

Original languageEnglish (US)
Pages (from-to)10681-10697
Number of pages17
JournalNucleic Acids Research
Volume16
Issue number22
DOIs
StatePublished - Nov 25 1988

Fingerprint

Ficusin
DNA-Directed RNA Polymerases
RNA
Oligomers
Promoter
Transcription
DNA
Interaction
Crosslinking
Template
Trioxsalen
Coding
Tin
bacteriophage T7 RNA polymerase
Mm

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)
  • Genetics

Cite this

Interaction of psoralen-derivatlzed oligodeoxyribonucleoside methyiphosphonates with synthetic DNA containing a promoter for 11 RNA polymerase. / Lee, Bok L.; Blake, Kathleen; Miller, Paul S.

In: Nucleic Acids Research, Vol. 16, No. 22, 25.11.1988, p. 10681-10697.

Research output: Contribution to journalArticle

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