CTTCCT-(T)4-TCCTTCAGCACA and target d-GTTTGTGCTGAAGGACACAC. The high Tm and hypochromicity of the triplex part of the chimera may be due to additional interactions outside the underlined binding site of the 20-mer. An intramolecular duplex/triDlex chimera is formed between d- sequences of the binding oligomer. Here we describe complex formation between a binding and target oligodeoxyribonucleotide which involves formation of a duplex/triplex chimera. A sequence at the 3'-end of the binding oligomer is complementary to and forms a duplex with a target sequence within the target oligomer. This target sequence contains a shorter homopurine tract which, under appropriate conditions, serves as a site for formation of an unusually stable triplex with a short homo-pyrimidine sequence at the 5'-end of the binding oligomer.
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