Interaction of oligodeoxyribonucleotides through formation of chimeric duplex/triplex complexes

David M. Noll; Jessica L. O’Rear; Cynthia D. Cushman; Paul S. Miller

doi:10.1080/15257779408011872

Interaction of oligodeoxyribonucleotides through formation of chimeric duplex/triplex complexes

David M. Noll, Jessica L. O’Rear, Cynthia D. Cushman, Paul S. Miller

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

CTTCCT-(T)₄-TCCTTCAGCACA and target d-GTTTGTGCTGAAGGACACAC. The high Tm and hypochromicity of the triplex part of the chimera may be due to additional interactions outside the underlined binding site of the 20-mer. An intramolecular duplex/triDlex chimera is formed between d- sequences of the binding oligomer. Here we describe complex formation between a binding and target oligodeoxyribonucleotide which involves formation of a duplex/triplex chimera. A sequence at the 3'-end of the binding oligomer is complementary to and forms a duplex with a target sequence within the target oligomer. This target sequence contains a shorter homopurine tract which, under appropriate conditions, serves as a site for formation of an unusually stable triplex with a short homo-pyrimidine sequence at the 5'-end of the binding oligomer.

Original language	English (US)
Pages (from-to)	997-1005
Number of pages	9
Journal	Nucleosides and Nucleotides
Volume	13
Issue number	4
DOIs	https://doi.org/10.1080/15257779408011872
State	Published - May 1 1994
Externally published	Yes

ASJC Scopus subject areas

Biochemistry
Genetics

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10.1080/15257779408011872

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@article{306d22ea5d504205bba7a397f9195372,

title = "Interaction of oligodeoxyribonucleotides through formation of chimeric duplex/triplex complexes",

abstract = "CTTCCT-(T)4-TCCTTCAGCACA and target d-GTTTGTGCTGAAGGACACAC. The high Tm and hypochromicity of the triplex part of the chimera may be due to additional interactions outside the underlined binding site of the 20-mer. An intramolecular duplex/triDlex chimera is formed between d- sequences of the binding oligomer. Here we describe complex formation between a binding and target oligodeoxyribonucleotide which involves formation of a duplex/triplex chimera. A sequence at the 3'-end of the binding oligomer is complementary to and forms a duplex with a target sequence within the target oligomer. This target sequence contains a shorter homopurine tract which, under appropriate conditions, serves as a site for formation of an unusually stable triplex with a short homo-pyrimidine sequence at the 5'-end of the binding oligomer.",

author = "Noll, {David M.} and O{\textquoteright}Rear, {Jessica L.} and Cushman, {Cynthia D.} and Miller, {Paul S.}",

note = "Funding Information: ACKNOWLEDGEMENT These studies were supported by a grant form the National Institutes of Health, GM 45012. D.M.N was supported by a Training Grant from the National Institute of Environmental Health Sciences, 5T32 ES07141 and J.L.O. was supported by a Training Grant from the National Cancer Institute, 2T32 CAO9110.",

year = "1994",

month = may,

day = "1",

doi = "10.1080/15257779408011872",

language = "English (US)",

volume = "13",

pages = "997--1005",

journal = "Nucleosides and Nucleotides",

issn = "0732-8311",

publisher = "Taylor and Francis Ltd.",

number = "4",

}

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T1 - Interaction of oligodeoxyribonucleotides through formation of chimeric duplex/triplex complexes

AU - Noll, David M.

AU - O’Rear, Jessica L.

AU - Cushman, Cynthia D.

AU - Miller, Paul S.

N1 - Funding Information: ACKNOWLEDGEMENT These studies were supported by a grant form the National Institutes of Health, GM 45012. D.M.N was supported by a Training Grant from the National Institute of Environmental Health Sciences, 5T32 ES07141 and J.L.O. was supported by a Training Grant from the National Cancer Institute, 2T32 CAO9110.

PY - 1994/5/1

Y1 - 1994/5/1

N2 - CTTCCT-(T)4-TCCTTCAGCACA and target d-GTTTGTGCTGAAGGACACAC. The high Tm and hypochromicity of the triplex part of the chimera may be due to additional interactions outside the underlined binding site of the 20-mer. An intramolecular duplex/triDlex chimera is formed between d- sequences of the binding oligomer. Here we describe complex formation between a binding and target oligodeoxyribonucleotide which involves formation of a duplex/triplex chimera. A sequence at the 3'-end of the binding oligomer is complementary to and forms a duplex with a target sequence within the target oligomer. This target sequence contains a shorter homopurine tract which, under appropriate conditions, serves as a site for formation of an unusually stable triplex with a short homo-pyrimidine sequence at the 5'-end of the binding oligomer.

AB - CTTCCT-(T)4-TCCTTCAGCACA and target d-GTTTGTGCTGAAGGACACAC. The high Tm and hypochromicity of the triplex part of the chimera may be due to additional interactions outside the underlined binding site of the 20-mer. An intramolecular duplex/triDlex chimera is formed between d- sequences of the binding oligomer. Here we describe complex formation between a binding and target oligodeoxyribonucleotide which involves formation of a duplex/triplex chimera. A sequence at the 3'-end of the binding oligomer is complementary to and forms a duplex with a target sequence within the target oligomer. This target sequence contains a shorter homopurine tract which, under appropriate conditions, serves as a site for formation of an unusually stable triplex with a short homo-pyrimidine sequence at the 5'-end of the binding oligomer.

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AN - SCOPUS:0028316963

SN - 0732-8311

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JO - Nucleosides and Nucleotides

JF - Nucleosides and Nucleotides

IS - 4

ER -

Interaction of oligodeoxyribonucleotides through formation of chimeric duplex/triplex complexes

Abstract

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