Interaction of non-lytic β-lactams with penicillin-binding proteins in Streptococcus pneumoniae

R. Hakenbeck, S. Tornette, N. F. Adkinson

Research output: Contribution to journalArticle

Abstract

The monobactam aztreonam and the cephalosporin ceftazidime, β-lactam antibiotics that possess the same side chain R1, showed unusual effects on exponentially growing pneumococci compared to other β-lactams. Both antibiotics did not induce lysis even at concentrations up to 2 mg ml-1, values well above the respective MICs. However, morphological alterations and growth inhibition of the cells were observed at much lower concentrations. Binding to penicillin-binding proteins (PBPs) in vitro could be monitored directly by using anti-aztreonam antiserum and the Western blot technique. Both antibiotics showed high affinity for PBP 3, but had an extremely low affinity for PBP 2b. It is suggested that the failure to bind to PBP 2b is responsible for the failure to induce lysis in pneumococci.

Original languageEnglish (US)
Pages (from-to)755-760
Number of pages6
JournalJournal of General Microbiology
Volume133
Issue number3
StatePublished - Jan 1 1987
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology

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