Interaction of heat shock protein Cpn10 with the Cyclin E/CDK2 substrate nuclear protein ataxia-telangiectasia (NPAT) is involved in regulating histone transcription

Li Ling Zheng, Fei Ya Wang, Xiao Xia Cong, Yue Shen, Xi Sheng Rao, Dao Sheng Huang, Wei Fan, Peng Yi, Xin Bao Wang, Lei Zheng, Yi Ting Zhou, Yan Luo

Research output: Contribution to journalArticle

Abstract

Precise modulation of histone gene transcription is critical for cell cycle progression. As a direct substrate of Cyclin E/CDK2, nuclear protein ataxia-telangiectasia (NPAT) is a crucial factor in regulating histone transcription and cell cycle progression. Here we identified that Cpn10/HSPE, a 10-kDa heat shock protein, is a novel interacting partner of NPAT. A pool of Cpn10 is colocalized with NPAT foci during G1 and S phases in nuclei. Gain- and loss-of-function experiments unraveled an essential role of Cpn10 in histone transcription.Aconserved DLFD motif within Cpn10 was critical for targeting NPAT and modulating histone transcription. More importantly, knockdown of Cpn10 disrupted the focus formation of both NPAT and FADD-like interleukin-1β-converting enzyme-associated huge protein without affecting Coilin-positive Cajal bodies. Finally, Cpn10 is important for S phase progression and cell proliferation. Taken together, our finding revealed a novel role of Cpn10 in the spatial regulation of NPAT signaling and disclosed a previously unappreciated link between the heat shock protein and histone transcription regulation.

Original languageEnglish (US)
Pages (from-to)29290-29300
Number of pages11
JournalJournal of Biological Chemistry
Volume290
Issue number49
DOIs
StatePublished - Dec 4 2015

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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