Interaction of DNA polymerase a-primase with cellular replication protein A and SV40 T antigen

Irene Dornreiter, Lome F. Erdile, Ilka U. Gilbert, Dorothea Von Winkler, Thomas Kelly, Ellen Fanning

Research output: Contribution to journalArticle

Abstract

The purified human single-stranded DNA binding protein, replication protein A (RP-A), forms specific complexes with purified SV40 large T antigen and with purified DNA polymerase α-primase, as shown by ELISA and a modified immunoblotting technique. RP-A associated efficiently with the isolated primase, as well as with intact polymerase a-primase. The 70 kDa subunit of RP-A was sufficient for association with polymerase α-primase. Purified SV40 large T antigen bound to intact RP-A and to polymerase - primase, but not to any of the separated subunits of RP-A or to the isolated primase. These results suggest that the specific protein-protein interactions between RP-A, polymerase-primase and T antigen may play a role in the initiation of SV40 DNA replication.

Original languageEnglish (US)
Pages (from-to)769-776
Number of pages8
JournalEMBO Journal
Volume11
Issue number2
StatePublished - 1992
Externally publishedYes

Fingerprint

Replication Protein A
DNA Primase
Polyomavirus Transforming Antigens
DNA-Directed DNA Polymerase
Viral Tumor Antigens
DNA-Binding Proteins
DNA Replication
Immunoblotting
Proteins
Enzyme-Linked Immunosorbent Assay
DNA

Keywords

  • DNA polymerase α
  • Initiation of DNA replication
  • Protein-protein interactions
  • Single-strand DNA binding proteinSV40 T antigen

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Dornreiter, I., Erdile, L. F., Gilbert, I. U., Von Winkler, D., Kelly, T., & Fanning, E. (1992). Interaction of DNA polymerase a-primase with cellular replication protein A and SV40 T antigen. EMBO Journal, 11(2), 769-776.

Interaction of DNA polymerase a-primase with cellular replication protein A and SV40 T antigen. / Dornreiter, Irene; Erdile, Lome F.; Gilbert, Ilka U.; Von Winkler, Dorothea; Kelly, Thomas; Fanning, Ellen.

In: EMBO Journal, Vol. 11, No. 2, 1992, p. 769-776.

Research output: Contribution to journalArticle

Dornreiter, I, Erdile, LF, Gilbert, IU, Von Winkler, D, Kelly, T & Fanning, E 1992, 'Interaction of DNA polymerase a-primase with cellular replication protein A and SV40 T antigen', EMBO Journal, vol. 11, no. 2, pp. 769-776.
Dornreiter I, Erdile LF, Gilbert IU, Von Winkler D, Kelly T, Fanning E. Interaction of DNA polymerase a-primase with cellular replication protein A and SV40 T antigen. EMBO Journal. 1992;11(2):769-776.
Dornreiter, Irene ; Erdile, Lome F. ; Gilbert, Ilka U. ; Von Winkler, Dorothea ; Kelly, Thomas ; Fanning, Ellen. / Interaction of DNA polymerase a-primase with cellular replication protein A and SV40 T antigen. In: EMBO Journal. 1992 ; Vol. 11, No. 2. pp. 769-776.
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AU - Kelly, Thomas

AU - Fanning, Ellen

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