Interaction between the Purα and E2F-1 transcription factors

Nune Darbinian, Martyn K. White, Gary L. Gallia, Shohreh Amini, Jay Rappaport, Kamel Khalili

Research output: Contribution to journalArticlepeer-review

Abstract

Background: Cell proliferation is regulated by E2F-1 which facilitates expression of genes involved in entry into S-phase. Release of E2F-1 from binding partners, e.g. pRb, is critical for G1/S progression. However ectopic E2F-1 overexpression activates p53 and inhibits growth. Previously, the multifunctional Purα protein was found to bind to E2F-1 and inhibit E2F-1 transcriptional activity. Materials and Methods: Purα deletion mutants were assayed for: in vitro binding to E2F-1, inhibition of E2F-1-induced promoter activation and effects on cell proliferation. Two RNA species with specific binding to E2F-1 and Purα were analyzed for their effects on E2F-1/Purα binding and cell growth. Results: The N-terminal 72 amino acids of Purα were involved in E2F-1 binding, inhibition of promoter activation by E2F-1 and reversal of E2F-mediated growth inhibition. The RNA species disrupted Purα/E2F-1 interaction and affected cell growth. Conclusion: E2F-1/Purα interaction has a role in the control of cell proliferation.

Original languageEnglish (US)
Pages (from-to)2585-2594
Number of pages10
JournalAnticancer Research
Volume24
Issue number5 A
StatePublished - Sep 1 2004

Keywords

  • Cell cycle
  • E2F1
  • Growth inhibition
  • Purα
  • RNA association

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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