Interaction between the keratin cytoskeleton and eEF1Bγ affects protein synthesis in epithelial cells

Seyun Kim, Juliane Kellner, Chang Hun Lee, Pierre A. Coulombe

Research output: Contribution to journalArticle

Abstract

Eukaryotic elongation factor-1 (eEF1) is essential for peptide-chain elongation during translation. We report that its γ subunit (eEF1Bγ) specifically binds, and bundles, keratin intermediate filaments. Disrupting this interaction depresses translation by ∼20% and selectively increases 80S ribosomes in epithelial cells, an outcome recapitulated by RNA interference-mediated silencing of eEF1Bγ. These findings extend the emerging relationship between keratin proteins and the translational machinery.

Original languageEnglish (US)
Pages (from-to)982-983
Number of pages2
JournalNature structural & molecular biology
Volume14
Issue number10
DOIs
StatePublished - Oct 2007

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Keratins
Cytoskeleton
Epithelial Cells
Peptide Elongation Factor 1
Intermediate Filaments
RNA Interference
Ribosomes
Proteins
Peptides

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

Cite this

Interaction between the keratin cytoskeleton and eEF1Bγ affects protein synthesis in epithelial cells. / Kim, Seyun; Kellner, Juliane; Lee, Chang Hun; Coulombe, Pierre A.

In: Nature structural & molecular biology, Vol. 14, No. 10, 10.2007, p. 982-983.

Research output: Contribution to journalArticle

Kim, Seyun ; Kellner, Juliane ; Lee, Chang Hun ; Coulombe, Pierre A. / Interaction between the keratin cytoskeleton and eEF1Bγ affects protein synthesis in epithelial cells. In: Nature structural & molecular biology. 2007 ; Vol. 14, No. 10. pp. 982-983.
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