Interaction between the keratin cytoskeleton and eEF1Bγ affects protein synthesis in epithelial cells

Seyun Kim; Juliane Kellner; Chang Hun Lee; Pierre A. Coulombe

doi:10.1038/nsmb1301

Interaction between the keratin cytoskeleton and eEF1Bγ affects protein synthesis in epithelial cells

Seyun Kim, Juliane Kellner, Chang Hun Lee, Pierre A. Coulombe

Research output: Contribution to journal › Article › peer-review

39 Scopus citations

Abstract

Eukaryotic elongation factor-1 (eEF1) is essential for peptide-chain elongation during translation. We report that its γ subunit (eEF1Bγ) specifically binds, and bundles, keratin intermediate filaments. Disrupting this interaction depresses translation by ∼20% and selectively increases 80S ribosomes in epithelial cells, an outcome recapitulated by RNA interference-mediated silencing of eEF1Bγ. These findings extend the emerging relationship between keratin proteins and the translational machinery.

Original language	English (US)
Pages (from-to)	982-983
Number of pages	2
Journal	Nature Structural and Molecular Biology
Volume	14
Issue number	10
DOIs	https://doi.org/10.1038/nsmb1301
State	Published - Oct 2007
Externally published	Yes

ASJC Scopus subject areas

Structural Biology
Molecular Biology

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10.1038/nsmb1301

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title = "Interaction between the keratin cytoskeleton and eEF1Bγ affects protein synthesis in epithelial cells",

abstract = "Eukaryotic elongation factor-1 (eEF1) is essential for peptide-chain elongation during translation. We report that its γ subunit (eEF1Bγ) specifically binds, and bundles, keratin intermediate filaments. Disrupting this interaction depresses translation by ∼20% and selectively increases 80S ribosomes in epithelial cells, an outcome recapitulated by RNA interference-mediated silencing of eEF1Bγ. These findings extend the emerging relationship between keratin proteins and the translational machinery.",

author = "Seyun Kim and Juliane Kellner and Lee, {Chang Hun} and Coulombe, {Pierre A.}",

note = "Funding Information: We thank J. Lorsch, C. Parent, T. Kinzy, J. Dijk, D. Robinson and Y. Zheng for help. This work was supported by US National Institutes of Health grants AR42047 and AR44232 to P.A.C.",

year = "2007",

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AU - Kellner, Juliane

AU - Lee, Chang Hun

AU - Coulombe, Pierre A.

N1 - Funding Information: We thank J. Lorsch, C. Parent, T. Kinzy, J. Dijk, D. Robinson and Y. Zheng for help. This work was supported by US National Institutes of Health grants AR42047 and AR44232 to P.A.C.

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AB - Eukaryotic elongation factor-1 (eEF1) is essential for peptide-chain elongation during translation. We report that its γ subunit (eEF1Bγ) specifically binds, and bundles, keratin intermediate filaments. Disrupting this interaction depresses translation by ∼20% and selectively increases 80S ribosomes in epithelial cells, an outcome recapitulated by RNA interference-mediated silencing of eEF1Bγ. These findings extend the emerging relationship between keratin proteins and the translational machinery.

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Interaction between the keratin cytoskeleton and eEF1Bγ affects protein synthesis in epithelial cells

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