Inter- and intra-assay reproducibility of microplate alamar blue assay results for isoniazid, rifampicin, ethambutol, streptomycin, ciprofloxacin, and capreomycin drug susceptibility testing of Mycobacterium tuberculosis

Brian Leonard, Jorge Coronel, Mark Siedner, Louis Grandjean, Luz Caviedes, Pilar Navarro, Robert H Gilman, David A J Moore

Research output: Contribution to journalArticle

Abstract

The intersample and intrasample variability of the results obtained with the microplate Alamar blue assay for the indirect drug susceptibility testing of Mycobacterium tuberculosis was investigated. Between 1.2 and 8.5% of paired MICs differed by more than one twofold dilution, resulting in discordant susceptible-resistant designations at frequencies between 0.6% (rifampin) and 18.9% (ethambutol).

Original languageEnglish (US)
Pages (from-to)3526-3529
Number of pages4
JournalJournal of Clinical Microbiology
Volume46
Issue number10
DOIs
StatePublished - Oct 2008

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Capreomycin
Ethambutol
Isoniazid
Streptomycin
Rifampin
Ciprofloxacin
Mycobacterium tuberculosis
Pharmaceutical Preparations
resazurin

ASJC Scopus subject areas

  • Microbiology (medical)

Cite this

Inter- and intra-assay reproducibility of microplate alamar blue assay results for isoniazid, rifampicin, ethambutol, streptomycin, ciprofloxacin, and capreomycin drug susceptibility testing of Mycobacterium tuberculosis. / Leonard, Brian; Coronel, Jorge; Siedner, Mark; Grandjean, Louis; Caviedes, Luz; Navarro, Pilar; Gilman, Robert H; Moore, David A J.

In: Journal of Clinical Microbiology, Vol. 46, No. 10, 10.2008, p. 3526-3529.

Research output: Contribution to journalArticle

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