Inter- and intra-assay reproducibility of microplate alamar blue assay results for isoniazid, rifampicin, ethambutol, streptomycin, ciprofloxacin, and capreomycin drug susceptibility testing of Mycobacterium tuberculosis

Brian Leonard; Jorge Coronel; Mark Siedner; Louis Grandjean; Luz Caviedes; Pilar Navarro; Robert H. Gilman; David A.J. Moore

doi:10.1128/JCM.02083-07

Inter- and intra-assay reproducibility of microplate alamar blue assay results for isoniazid, rifampicin, ethambutol, streptomycin, ciprofloxacin, and capreomycin drug susceptibility testing of Mycobacterium tuberculosis

Brian Leonard, Jorge Coronel, Mark Siedner, Louis Grandjean, Luz Caviedes, Pilar Navarro, Robert H. Gilman, David A.J. Moore

Bloomberg School of Public Health

Research output: Contribution to journal › Article › peer-review

30 Scopus citations

Abstract

The intersample and intrasample variability of the results obtained with the microplate Alamar blue assay for the indirect drug susceptibility testing of Mycobacterium tuberculosis was investigated. Between 1.2 and 8.5% of paired MICs differed by more than one twofold dilution, resulting in discordant susceptible-resistant designations at frequencies between 0.6% (rifampin) and 18.9% (ethambutol).

Original language	English (US)
Pages (from-to)	3526-3529
Number of pages	4
Journal	Journal of clinical microbiology
Volume	46
Issue number	10
DOIs	https://doi.org/10.1128/JCM.02083-07
State	Published - Oct 2008

ASJC Scopus subject areas

Microbiology (medical)

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Leonard, B., Coronel, J., Siedner, M., Grandjean, L., Caviedes, L., Navarro, P., Gilman, R. H., & Moore, D. A. J. (2008). Inter- and intra-assay reproducibility of microplate alamar blue assay results for isoniazid, rifampicin, ethambutol, streptomycin, ciprofloxacin, and capreomycin drug susceptibility testing of Mycobacterium tuberculosis. Journal of clinical microbiology, 46(10), 3526-3529. https://doi.org/10.1128/JCM.02083-07

Inter- and intra-assay reproducibility of microplate alamar blue assay results for isoniazid, rifampicin, ethambutol, streptomycin, ciprofloxacin, and capreomycin drug susceptibility testing of Mycobacterium tuberculosis. / Leonard, Brian; Coronel, Jorge; Siedner, Mark et al.
In: Journal of clinical microbiology, Vol. 46, No. 10, 10.2008, p. 3526-3529.

Research output: Contribution to journal › Article › peer-review

Leonard, B, Coronel, J, Siedner, M, Grandjean, L, Caviedes, L, Navarro, P, Gilman, RH & Moore, DAJ 2008, 'Inter- and intra-assay reproducibility of microplate alamar blue assay results for isoniazid, rifampicin, ethambutol, streptomycin, ciprofloxacin, and capreomycin drug susceptibility testing of Mycobacterium tuberculosis', Journal of clinical microbiology, vol. 46, no. 10, pp. 3526-3529. https://doi.org/10.1128/JCM.02083-07

Leonard B, Coronel J, Siedner M, Grandjean L, Caviedes L, Navarro P et al. Inter- and intra-assay reproducibility of microplate alamar blue assay results for isoniazid, rifampicin, ethambutol, streptomycin, ciprofloxacin, and capreomycin drug susceptibility testing of Mycobacterium tuberculosis. Journal of clinical microbiology. 2008 Oct;46(10):3526-3529. doi: 10.1128/JCM.02083-07

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Inter- and intra-assay reproducibility of microplate alamar blue assay results for isoniazid, rifampicin, ethambutol, streptomycin, ciprofloxacin, and capreomycin drug susceptibility testing of Mycobacterium tuberculosis

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