Integrin Molecular Tension within Motile Focal Adhesions

Xuefeng Wang, Jie Sun, Qian Xu, Farhan Chowdhury, Mehdi Roein-Peikar, Yingxiao Wang, Taekjip Ha

Research output: Contribution to journalArticle

Abstract

Forces transmitted by integrins regulate many important cellular functions. Previously, we developed tension gauge tether (TGT) as a molecular force sensor and determined the threshold tension across a single integrin-ligand bond, termed integrin tension, required for initial cell adhesion. Here, we used fluorescently labeled TGTs to study the magnitude and spatial distribution of integrin tension on the cell-substratum interface. We observed two distinct levels of integrin tension. A >54 pN molecular tension is transmitted by clustered integrins in motile focal adhesions (FAs) and such force is generated by actomyosin, whereas the previously reported ∼40 pN integrin tension is transmitted by integrins before FA formation and is independent of actomyosin. We then studied FA motility using a TGT-coated surface as a fluorescent canvas, which records the history of integrin force activity. Our data suggest that the region of the strongest integrin force overlaps with the center of a motile FA within 0.2 μm resolution. We also found that FAs move in pairs and that the asymmetry in the motility of an FA pair is dependent on the initial FA locations on the cell-substratum interface.

Original languageEnglish (US)
Pages (from-to)2259-2267
Number of pages9
JournalBiophysical Journal
Volume109
Issue number11
DOIs
StatePublished - Dec 1 2015

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Focal Adhesions
Integrins
Actomyosin
Cell Adhesion
History
Ligands

ASJC Scopus subject areas

  • Biophysics

Cite this

Wang, X., Sun, J., Xu, Q., Chowdhury, F., Roein-Peikar, M., Wang, Y., & Ha, T. (2015). Integrin Molecular Tension within Motile Focal Adhesions. Biophysical Journal, 109(11), 2259-2267. https://doi.org/10.1016/j.bpj.2015.10.029

Integrin Molecular Tension within Motile Focal Adhesions. / Wang, Xuefeng; Sun, Jie; Xu, Qian; Chowdhury, Farhan; Roein-Peikar, Mehdi; Wang, Yingxiao; Ha, Taekjip.

In: Biophysical Journal, Vol. 109, No. 11, 01.12.2015, p. 2259-2267.

Research output: Contribution to journalArticle

Wang, X, Sun, J, Xu, Q, Chowdhury, F, Roein-Peikar, M, Wang, Y & Ha, T 2015, 'Integrin Molecular Tension within Motile Focal Adhesions', Biophysical Journal, vol. 109, no. 11, pp. 2259-2267. https://doi.org/10.1016/j.bpj.2015.10.029
Wang X, Sun J, Xu Q, Chowdhury F, Roein-Peikar M, Wang Y et al. Integrin Molecular Tension within Motile Focal Adhesions. Biophysical Journal. 2015 Dec 1;109(11):2259-2267. https://doi.org/10.1016/j.bpj.2015.10.029
Wang, Xuefeng ; Sun, Jie ; Xu, Qian ; Chowdhury, Farhan ; Roein-Peikar, Mehdi ; Wang, Yingxiao ; Ha, Taekjip. / Integrin Molecular Tension within Motile Focal Adhesions. In: Biophysical Journal. 2015 ; Vol. 109, No. 11. pp. 2259-2267.
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