Abstract
The concept of one-protein-multiple-function, i.e. moonlighting proteins, is an ever-expanding paradigm. We obtained compelling evidence that an array of 'cytoplasmic' metabolic enzymes can enter the nuclei to carry out moonlighting transcription functions; this phenomenon is conserved from Drosophila to humans. Of particular interest are the classical glycolytic enzymes GAPDH (glyceraldehyde-3-phosphate dehydrogenase) and LDH (lactate dehydrogenase), which utilize NAD(H) as coenzymes and not only moonlight (in their nuclear forms) to regulate the transcription of S-phase-specific histone genes, but also act as metabolic/redox sensors that link histone gene switching to DNA replication and S-phase progression.
Original language | English (US) |
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Article number | e00018 |
Pages (from-to) | 187-197 |
Number of pages | 11 |
Journal | Bioscience Reports |
Volume | 33 |
Issue number | 2 |
DOIs | |
State | Published - 2013 |
Keywords
- Chromosome
- Enzyme
- GAPDH
- Histone 2B
- S-phase
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology