Integration of the metabolic/redox state, histone gene switching, DNA replication and S-phase progression by moonlighting metabolic enzymes

Hongpeng He, Mei Chin Lee, Li Ling Zheng, Lei Zheng, Yan Luo

Research output: Contribution to journalArticle


The concept of one-protein-multiple-function, i.e. moonlighting proteins, is an ever-expanding paradigm. We obtained compelling evidence that an array of 'cytoplasmic' metabolic enzymes can enter the nuclei to carry out moonlighting transcription functions; this phenomenon is conserved from Drosophila to humans. Of particular interest are the classical glycolytic enzymes GAPDH (glyceraldehyde-3-phosphate dehydrogenase) and LDH (lactate dehydrogenase), which utilize NAD(H) as coenzymes and not only moonlight (in their nuclear forms) to regulate the transcription of S-phase-specific histone genes, but also act as metabolic/redox sensors that link histone gene switching to DNA replication and S-phase progression.

Original languageEnglish (US)
Article numbere00018
Pages (from-to)187-197
Number of pages11
JournalBioscience Reports
Issue number2
StatePublished - 2013


  • Chromosome
  • Enzyme
  • Histone 2B
  • S-phase

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

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