Integration of Phosphoinositide- and Calmodulin-Mediated Regulation of TRPC6

Young Kwon, Thomas Hofmann, Craig Montell

Research output: Contribution to journalArticlepeer-review

Abstract

Multiple TRP channels are regulated by phosphoinositides (PIs). However, it is not known whether PIs bind directly to TRP channels. Furthermore, the mechanisms through which PIs regulate TRP channels are obscure. To analyze the role of PI/TRP interactions, we used a biochemical approach, focusing on TRPC6. TRPC6 bound directly to PIs, and with highest potency to phosphatidylinositol 3,4,5-trisphosphate (PIP3). We found that PIP3 binding disrupted the association of calmodulin (CaM) with TRPC6. We identified the PIP3-binding site and found that mutations that increased or decreased the affinity of the PIP3/TRPC6 interaction enhanced or reduced the TRPC6-dependent current, respectively. PI-mediated disruption of CaM binding appears to be a theme that applies to other TRP channels, such as TRPV1, as well as to the voltage-gated channels KCNQ1 and Cav1.2. We propose that regulation of CaM binding by PIs provides a mode for integration of channel regulation by Ca2+ and PIs.

Original languageEnglish (US)
Pages (from-to)491-503
Number of pages13
JournalMolecular Cell
Volume25
Issue number4
DOIs
StatePublished - Feb 23 2007

Keywords

  • SIGNALING

ASJC Scopus subject areas

  • Molecular Biology

Fingerprint

Dive into the research topics of 'Integration of Phosphoinositide- and Calmodulin-Mediated Regulation of TRPC6'. Together they form a unique fingerprint.

Cite this