Integration of diffraction phase microscopy and Raman imaging for label-free morpho-molecular assessment of live cells

Rishikesh Pandey, Renjie Zhou, Rosalie Bordett, Ciera Hunter, Kristine Glunde, Ishan Barman, Tulio Valdez, Christine Finck

Research output: Contribution to journalArticlepeer-review

Abstract

Label-free quantitative imaging is highly desirable for studying live cells by extracting pathophysiological information without perturbing cell functions. Here, we demonstrate a novel label-free multimodal optical imaging system with the capability of providing comprehensive morphological and molecular attributes of live cells. Our morpho-molecular microscopy (3M) system draws on the combined strength of quantitative phase microscopy (QPM) and Raman microscopy to probe the morphological features and molecular fingerprinting characteristics of each cell under observation. While the commonr-path geometry of our QPM system allows for highly sensitive phase measurement, the Raman microscopy is equipped with dual excitation wavelengths and utilizes the same detection and dispersion system, making it a distinctive multi-wavelength system with a small footprint. We demonstrate the applicability of the 3M system by investigating nucleated and nonnucleated cells. This integrated label-free platform has a promising potential in preclinical research, as well as in clinical diagnosis in the near future.

Original languageEnglish (US)
Article numbere201800291
JournalJournal of biophotonics
Volume12
Issue number4
DOIs
StatePublished - Apr 2019

Keywords

  • Raman spectroscopy
  • cellular imaging
  • label-free imaging
  • quantitative phase microscopy

ASJC Scopus subject areas

  • Chemistry(all)
  • Materials Science(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Engineering(all)
  • Physics and Astronomy(all)

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