Integration and regression of implanted engineered human vascular networks during deep wound healing

Donny Hanjaya-Putra, YU I. Shen, Abigail Wilson, Karen Fox-Talbot, Sudhir Khetan, Jason A. Burdick, Charles Steenbergen, Sharon Gerecht

Research output: Contribution to journalArticlepeer-review


The ability of vascularized constructs to integrate with tissues may depend on the kinetics and stability of vascular structure development. This study assessed the functionality and durability of engineered human vasculatures from endothelial progenitors when implanted in a mouse deep burn-wound model. Human vascular networks, derived from endothelial colony-forming cells in hyaluronic acid hydrogels, were transplanted into third-degree burns. On day 3 following transplantation, macrophages rapidly degraded the hydrogel during a period of inflammation; through the transitions from inflammation to proliferation (days 5-7), the host's vasculatures infiltrated the construct, connecting with the human vessels within the wound area. The growth of mouse vessels near the wound area supported further integration with the implanted human vasculatures. During this period, the majority of the vessels (~60%) in the treated wound area were human. Although no increase in the density of human vessels was detected during the proliferative phase, they temporarily increased in size. This growth peaked at day 7, the middle of the proliferation stage, and then decreased by the end of the proliferation stage. As the wound reached the remodeling period during the second week after transplantation, the vasculatures including the transplanted human vessels generally regressed, and few microvessels, wrapped by mouse smooth muscle cells and with a vessel area less than 200 μm2 (including the human ones), remained in the healed wound. Overall, this study offers useful insights for the development of vascularization strategies for wound healing and ischemic conditions, for tissue-engineered constructs, and for tissue regeneration.

Original languageEnglish (US)
Pages (from-to)297-306
Number of pages10
JournalStem Cells Translational Medicine
Issue number4
StatePublished - 2013


  • Angiogenesis and bull
  • Endothelial progenitors and bull
  • Hyaluronan and bull
  • Hydrogel and bull
  • Microvasculature
  • Tissue regeneration and bull
  • Vascular engineering and bull
  • Wound healing and bull

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology


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