Integration and application of vitrified collagen in multilayered microfluidic devices for corneal microtissue culture

Christopher M. Puleo, Winnette McIntosh Ambrose, Toshiaki Takezawa, Jennifer Hartt Elisseeff, Tza Huei Wang

Research output: Contribution to journalArticle

Abstract

This paper describes the fabrication and application of microfluidic devices containing collagen vitrigel (CV) used as both a functional and sacrificial cell growth substrate for the development of corneal microtissue patches. Within the device, vacuum fixation of the CV in a dehydrated state enables quick integration with standard multilayer soft lithographic techniques, while on-chip rehydration results in a gel-like collagen substrate for microfluidic cell culture. Fluidic connectivity to both the apical and basal side of the CV permits bilayered culture of epithelium and supporting stromal cell layers. In addition, microfluidic introduction of a collagenase etching media enables sacrificial degradation of the supporting CV membrane for development of barrier tissue constructs containing minimal synthetic substrate. The utility of this platform was evaluated by miniaturizing the standard transepithelial permeability (TEP) assay in order to measure the integrity of an array of corneal tissue micropatches.

Original languageEnglish (US)
Pages (from-to)3221-3227
Number of pages7
JournalLab on a Chip - Miniaturisation for Chemistry and Biology
Volume9
Issue number22
DOIs
StatePublished - 2009

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Lab-On-A-Chip Devices
Microfluidics
Collagen
Cell culture
Substrates
Tissue
Fluid Therapy
Cell growth
Fluidics
Collagenases
Stromal Cells
Vacuum
Growth and Development
Etching
Permeability
Assays
Multilayers
Gels
Epithelium
Cell Culture Techniques

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)
  • Bioengineering
  • Biomedical Engineering

Cite this

Integration and application of vitrified collagen in multilayered microfluidic devices for corneal microtissue culture. / Puleo, Christopher M.; McIntosh Ambrose, Winnette; Takezawa, Toshiaki; Elisseeff, Jennifer Hartt; Wang, Tza Huei.

In: Lab on a Chip - Miniaturisation for Chemistry and Biology, Vol. 9, No. 22, 2009, p. 3221-3227.

Research output: Contribution to journalArticle

Puleo, Christopher M. ; McIntosh Ambrose, Winnette ; Takezawa, Toshiaki ; Elisseeff, Jennifer Hartt ; Wang, Tza Huei. / Integration and application of vitrified collagen in multilayered microfluidic devices for corneal microtissue culture. In: Lab on a Chip - Miniaturisation for Chemistry and Biology. 2009 ; Vol. 9, No. 22. pp. 3221-3227.
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