Integrated miRNA profiling and bioinformatics analyses reveal potential causative miRNAs in gastric adenocarcinoma

Xiaojing Zhang, Yin Peng, Zhe Jin, Weiling Huang, Yulan Cheng, Yudan Liu, Xianling Feng, Mengting Yang, Yong Huang, Zhenfu Zhao, Liang Wang, Yanjie Wei, Xinmin Fan, Duo Zheng, Stephen J. Meltzer

Research output: Contribution to journalArticlepeer-review

Abstract

Gastric cancer (GC) is one of the leading causes of cancer-related deaths throughout China and worldwide. The discovery of microRNAs (miRNAs) has provided a new opportunity for developing diagnostic biomarkers and effective therapeutic targets in GC. By performing microarray analyses of benign and malignant gastric epithelial cell lines (HFE145, NCI-N87, MKN28, RF1, KATO III and RF48), 16 significantly dysregulated miRNAs were found. 11 of these were validated by real-time qRT-PCR. Based on miRWalk online database scans, 703 potential mRNA targets of the 16 miRNAs were identified. Bioinformatic analyses suggested that these dysregulated miRNAs and their predicted targets were principally involved in tumor pathogenesis, MAPK signaling, and apoptosis. Finally, miRNA-gene network analyses identified miRNA-125b as a crucial miRNA in GC development. Taken together, these results develop a comprehensive expression and functional profile of differentially expressed miRNAs related to gastric oncogenesis. This profile may serve as a potential tool for biomarker and therapeutic target identification in GC patients.

Original languageEnglish (US)
Pages (from-to)32878-32889
Number of pages12
JournalOncotarget
Volume6
Issue number32
DOIs
StatePublished - 2015

Keywords

  • Bioinformatics
  • Gastric cancer
  • MiRNA profiling

ASJC Scopus subject areas

  • Oncology

Fingerprint Dive into the research topics of 'Integrated miRNA profiling and bioinformatics analyses reveal potential causative miRNAs in gastric adenocarcinoma'. Together they form a unique fingerprint.

Cite this