Integrated miRNA profiling and bioinformatics analyses reveal potential causative miRNAs in gastric adenocarcinoma

Xiaojing Zhang; Yin Peng; Zhe Jin; Weiling Huang; Yulan Cheng; Yudan Liu; Xianling Feng; Mengting Yang; Yong Huang; Zhenfu Zhao; Liang Wang; Yanjie Wei; Xinmin Fan; Duo Zheng; Stephen J. Meltzer

doi:10.18632/oncotarget.5419

Integrated miRNA profiling and bioinformatics analyses reveal potential causative miRNAs in gastric adenocarcinoma

Xiaojing Zhang, Yin Peng, Zhe Jin, Weiling Huang, Yulan Cheng, Yudan Liu, Xianling Feng, Mengting Yang, Yong Huang, Zhenfu Zhao, Liang Wang, Yanjie Wei, Xinmin Fan, Duo Zheng, Stephen J. Meltzer

School of Medicine

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

Gastric cancer (GC) is one of the leading causes of cancer-related deaths throughout China and worldwide. The discovery of microRNAs (miRNAs) has provided a new opportunity for developing diagnostic biomarkers and effective therapeutic targets in GC. By performing microarray analyses of benign and malignant gastric epithelial cell lines (HFE145, NCI-N87, MKN28, RF1, KATO III and RF48), 16 significantly dysregulated miRNAs were found. 11 of these were validated by real-time qRT-PCR. Based on miRWalk online database scans, 703 potential mRNA targets of the 16 miRNAs were identified. Bioinformatic analyses suggested that these dysregulated miRNAs and their predicted targets were principally involved in tumor pathogenesis, MAPK signaling, and apoptosis. Finally, miRNA-gene network analyses identified miRNA-125b as a crucial miRNA in GC development. Taken together, these results develop a comprehensive expression and functional profile of differentially expressed miRNAs related to gastric oncogenesis. This profile may serve as a potential tool for biomarker and therapeutic target identification in GC patients.

Original language	English (US)
Pages (from-to)	32878-32889
Number of pages	12
Journal	Oncotarget
Volume	6
Issue number	32
DOIs	https://doi.org/10.18632/oncotarget.5419
State	Published - 2015

Keywords

Bioinformatics
Gastric cancer
MiRNA profiling

ASJC Scopus subject areas

Oncology

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title = "Integrated miRNA profiling and bioinformatics analyses reveal potential causative miRNAs in gastric adenocarcinoma",

abstract = "Gastric cancer (GC) is one of the leading causes of cancer-related deaths throughout China and worldwide. The discovery of microRNAs (miRNAs) has provided a new opportunity for developing diagnostic biomarkers and effective therapeutic targets in GC. By performing microarray analyses of benign and malignant gastric epithelial cell lines (HFE145, NCI-N87, MKN28, RF1, KATO III and RF48), 16 significantly dysregulated miRNAs were found. 11 of these were validated by real-time qRT-PCR. Based on miRWalk online database scans, 703 potential mRNA targets of the 16 miRNAs were identified. Bioinformatic analyses suggested that these dysregulated miRNAs and their predicted targets were principally involved in tumor pathogenesis, MAPK signaling, and apoptosis. Finally, miRNA-gene network analyses identified miRNA-125b as a crucial miRNA in GC development. Taken together, these results develop a comprehensive expression and functional profile of differentially expressed miRNAs related to gastric oncogenesis. This profile may serve as a potential tool for biomarker and therapeutic target identification in GC patients.",

keywords = "Bioinformatics, Gastric cancer, MiRNA profiling",

author = "Xiaojing Zhang and Yin Peng and Zhe Jin and Weiling Huang and Yulan Cheng and Yudan Liu and Xianling Feng and Mengting Yang and Yong Huang and Zhenfu Zhao and Liang Wang and Yanjie Wei and Xinmin Fan and Duo Zheng and Meltzer, {Stephen J.}",

year = "2015",

doi = "10.18632/oncotarget.5419",

language = "English (US)",

volume = "6",

pages = "32878--32889",

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T1 - Integrated miRNA profiling and bioinformatics analyses reveal potential causative miRNAs in gastric adenocarcinoma

AU - Zhang, Xiaojing

AU - Peng, Yin

AU - Jin, Zhe

AU - Huang, Weiling

AU - Cheng, Yulan

AU - Liu, Yudan

AU - Feng, Xianling

AU - Yang, Mengting

AU - Huang, Yong

AU - Zhao, Zhenfu

AU - Wang, Liang

AU - Wei, Yanjie

AU - Fan, Xinmin

AU - Zheng, Duo

AU - Meltzer, Stephen J.

PY - 2015

Y1 - 2015

N2 - Gastric cancer (GC) is one of the leading causes of cancer-related deaths throughout China and worldwide. The discovery of microRNAs (miRNAs) has provided a new opportunity for developing diagnostic biomarkers and effective therapeutic targets in GC. By performing microarray analyses of benign and malignant gastric epithelial cell lines (HFE145, NCI-N87, MKN28, RF1, KATO III and RF48), 16 significantly dysregulated miRNAs were found. 11 of these were validated by real-time qRT-PCR. Based on miRWalk online database scans, 703 potential mRNA targets of the 16 miRNAs were identified. Bioinformatic analyses suggested that these dysregulated miRNAs and their predicted targets were principally involved in tumor pathogenesis, MAPK signaling, and apoptosis. Finally, miRNA-gene network analyses identified miRNA-125b as a crucial miRNA in GC development. Taken together, these results develop a comprehensive expression and functional profile of differentially expressed miRNAs related to gastric oncogenesis. This profile may serve as a potential tool for biomarker and therapeutic target identification in GC patients.

AB - Gastric cancer (GC) is one of the leading causes of cancer-related deaths throughout China and worldwide. The discovery of microRNAs (miRNAs) has provided a new opportunity for developing diagnostic biomarkers and effective therapeutic targets in GC. By performing microarray analyses of benign and malignant gastric epithelial cell lines (HFE145, NCI-N87, MKN28, RF1, KATO III and RF48), 16 significantly dysregulated miRNAs were found. 11 of these were validated by real-time qRT-PCR. Based on miRWalk online database scans, 703 potential mRNA targets of the 16 miRNAs were identified. Bioinformatic analyses suggested that these dysregulated miRNAs and their predicted targets were principally involved in tumor pathogenesis, MAPK signaling, and apoptosis. Finally, miRNA-gene network analyses identified miRNA-125b as a crucial miRNA in GC development. Taken together, these results develop a comprehensive expression and functional profile of differentially expressed miRNAs related to gastric oncogenesis. This profile may serve as a potential tool for biomarker and therapeutic target identification in GC patients.

KW - Bioinformatics

KW - Gastric cancer

KW - MiRNA profiling

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Integrated miRNA profiling and bioinformatics analyses reveal potential causative miRNAs in gastric adenocarcinoma

Abstract

Keywords

ASJC Scopus subject areas

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