TY - JOUR
T1 - Inteferon gamma and granulocyte-macrophage colony-stimulating factor augment the antifungal activity of human polymorphonuclear leukocytes against Scedosporium spp.
T2 - Comparison with Aspergillus spp
AU - Gil-Lamaignere, Cristina
AU - Winn, Richard M.
AU - Simitsopoulou, Maria
AU - Maloukou, Avgi
AU - Walsh, Thomas J.
AU - Roilides, Emmanuel
N1 - Funding Information:
C.G.L. and R.M.W. were research fellows supported by the European Commission Training and Mobility of Researchers (grant FMRX-CT970145 Eurofung).
PY - 2005/5
Y1 - 2005/5
N2 - While Aspergillus spp. have been the most frequent filamentous fungi causing infections in immunocompromised patients, Scedosporium spp. are emerging as life-threatening pathogens. We studied the effects of interferon gamma (IFN-γ) and granulocyte-macrophage colony-stimulating factor (GM-CSF) alone or combined on the antifungal activities of human polymorphonuclear leukocytes (PMN) against Scedosporium apiospermum and Scedosporium prolificans. We paralleled these activities to those against Aspergillus fumigatus and Aspergillus flavus. Incubation of PMN with IFN-γ and GM-CSF for 22 h enhanced PMN-induced hyphal damage of both Aspergillus spp. and S. prolificans (p < 0.05) but not of S. apiospermum. However, hyphae of S. apiospermum were damaged significantly more after incubation with PMN that had been treated with IFN-γ and GM-CSF for 2 h. In addition, incubation of PMN with GM-CSF for 2 h enhanced PMN oxidative burst measured as superoxide anion (O2-) production in response to non-opsonized hyphae of A. flavus and Scedosporium spp. (p < 0.05). In contrast, after 2 h, IFN-γ and GM-CSF alone did not enhance PMN O2- in response to opsonized hyphae of A. flavus and Scedosporium spp.; however, the combination of IFN-γ and GM-CSF showed significant enhancement against these species. Thus, IFN-γ and GM-CSF, particularly in combination, demonstrate a species- and time-dependent augmentation of PMN responses to Scedosporium spp.
AB - While Aspergillus spp. have been the most frequent filamentous fungi causing infections in immunocompromised patients, Scedosporium spp. are emerging as life-threatening pathogens. We studied the effects of interferon gamma (IFN-γ) and granulocyte-macrophage colony-stimulating factor (GM-CSF) alone or combined on the antifungal activities of human polymorphonuclear leukocytes (PMN) against Scedosporium apiospermum and Scedosporium prolificans. We paralleled these activities to those against Aspergillus fumigatus and Aspergillus flavus. Incubation of PMN with IFN-γ and GM-CSF for 22 h enhanced PMN-induced hyphal damage of both Aspergillus spp. and S. prolificans (p < 0.05) but not of S. apiospermum. However, hyphae of S. apiospermum were damaged significantly more after incubation with PMN that had been treated with IFN-γ and GM-CSF for 2 h. In addition, incubation of PMN with GM-CSF for 2 h enhanced PMN oxidative burst measured as superoxide anion (O2-) production in response to non-opsonized hyphae of A. flavus and Scedosporium spp. (p < 0.05). In contrast, after 2 h, IFN-γ and GM-CSF alone did not enhance PMN O2- in response to opsonized hyphae of A. flavus and Scedosporium spp.; however, the combination of IFN-γ and GM-CSF showed significant enhancement against these species. Thus, IFN-γ and GM-CSF, particularly in combination, demonstrate a species- and time-dependent augmentation of PMN responses to Scedosporium spp.
KW - Aspergillus spp.
KW - Hyphal damage
KW - Oxidative burst
KW - Polymorphonuclear leukocyte
KW - Scedosporium spp.
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U2 - 10.1080/13693780412331271072
DO - 10.1080/13693780412331271072
M3 - Article
C2 - 16010852
AN - SCOPUS:20944444512
SN - 1369-3786
VL - 43
SP - 253
EP - 260
JO - Medical mycology
JF - Medical mycology
IS - 3
ER -