Inteferon gamma and granulocyte-macrophage colony-stimulating factor augment the antifungal activity of human polymorphonuclear leukocytes against Scedosporium spp.

Comparison with Aspergillus spp

Cristina Gil-Lamaignere, Richard M. Winn, Maria Simitsopoulou, Avgi Maloukou, Thomas J. Walsh, Emmanuel Roilides

Research output: Contribution to journalArticle

Abstract

While Aspergillus spp. have been the most frequent filamentous fungi causing infections in immunocompromised patients, Scedosporium spp. are emerging as life-threatening pathogens. We studied the effects of interferon gamma (IFN-γ) and granulocyte-macrophage colony-stimulating factor (GM-CSF) alone or combined on the antifungal activities of human polymorphonuclear leukocytes (PMN) against Scedosporium apiospermum and Scedosporium prolificans. We paralleled these activities to those against Aspergillus fumigatus and Aspergillus flavus. Incubation of PMN with IFN-γ and GM-CSF for 22 h enhanced PMN-induced hyphal damage of both Aspergillus spp. and S. prolificans (p <0.05) but not of S. apiospermum. However, hyphae of S. apiospermum were damaged significantly more after incubation with PMN that had been treated with IFN-γ and GM-CSF for 2 h. In addition, incubation of PMN with GM-CSF for 2 h enhanced PMN oxidative burst measured as superoxide anion (O2-) production in response to non-opsonized hyphae of A. flavus and Scedosporium spp. (p <0.05). In contrast, after 2 h, IFN-γ and GM-CSF alone did not enhance PMN O2- in response to opsonized hyphae of A. flavus and Scedosporium spp.; however, the combination of IFN-γ and GM-CSF showed significant enhancement against these species. Thus, IFN-γ and GM-CSF, particularly in combination, demonstrate a species- and time-dependent augmentation of PMN responses to Scedosporium spp.

Original languageEnglish (US)
Pages (from-to)253-260
Number of pages8
JournalMedical Mycology
Volume43
Issue number3
DOIs
StatePublished - May 2005
Externally publishedYes

Fingerprint

Scedosporium
granulocyte-macrophage colony-stimulating factor
Aspergillus
Granulocyte-Macrophage Colony-Stimulating Factor
Human Activities
neutrophils
Neutrophils
interferon-gamma
Interferon-gamma
Aspergillus flavus
Hyphae
hyphae
Aspergillus fumigatus
Respiratory Burst
Mycoses
Immunocompromised Host
Interferon-alpha
Superoxides
superoxide anion
Fungi

Keywords

  • Aspergillus spp.
  • Hyphal damage
  • Oxidative burst
  • Polymorphonuclear leukocyte
  • Scedosporium spp.

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • veterinary(all)
  • Microbiology

Cite this

Inteferon gamma and granulocyte-macrophage colony-stimulating factor augment the antifungal activity of human polymorphonuclear leukocytes against Scedosporium spp. Comparison with Aspergillus spp. / Gil-Lamaignere, Cristina; Winn, Richard M.; Simitsopoulou, Maria; Maloukou, Avgi; Walsh, Thomas J.; Roilides, Emmanuel.

In: Medical Mycology, Vol. 43, No. 3, 05.2005, p. 253-260.

Research output: Contribution to journalArticle

Gil-Lamaignere, Cristina ; Winn, Richard M. ; Simitsopoulou, Maria ; Maloukou, Avgi ; Walsh, Thomas J. ; Roilides, Emmanuel. / Inteferon gamma and granulocyte-macrophage colony-stimulating factor augment the antifungal activity of human polymorphonuclear leukocytes against Scedosporium spp. Comparison with Aspergillus spp. In: Medical Mycology. 2005 ; Vol. 43, No. 3. pp. 253-260.
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