Insulin-stimulated exocytosis of GLUT4 is enhanced by IRAP and its partner tankyrase

Tsung Yin J Yeh, Juan Sbodio, Zhi Yang Tsun, Biao Luo, Nai Wen Chi

Research output: Contribution to journalArticle

Abstract

The glucose transporter GLUT4 and the aminopeptidase IRAP (insulin-responsive aminopeptidase) are the major cargo proteins of GSVs (GLUT4 storage vesicles) in adipocytes and myocytes. In the basal state, most GSVs are sequestered in perinuclear and other cytosolic compartments. Following insulin stimulation, GSVs undergo exocytic translocation to insert GLUT4 and IRAP into the plasma membrane. The mechanisms regulating GSV trafficking are not fully defined. In the present study, using 3T3-L1 adipocytes transfected with siRNAs (small interfering RNAs), we show that insulin-stimulated IRAP translocation remained intact despite substantial GLUT4 knockdown. By contrast, insulin-stimulated GLUT4 translocation was impaired upon IRAP knockdown, indicating that IRAP plays a role in GSV trafficking. We also show that knockdown of tankyrase, a Golgi-associated IRAP-binding protein that co-localizes with perinuclear GSVs, attenuated insulin-stimulated GSV translocation and glucose uptake without disrupting insulin-induced phosphorylation cascades. Moreover, iodixanol density gradient analyses revealed that tankyrase knockdown altered the basal-state partitioning of GLUT4 and IRAP within endosomal compartments, apparently by shifting both proteins toward less buoyant compartments. Importantly, the afore-mentioned effects of tankyrase knockdown were reproduced by treating adipocytes with PJ34, a general PARP (poly-ADP-ribose polymerase) inhibitor that abrogated tankyrase-mediated protein modification known as poly-ADP-ribosylation. Collectively, these findings suggest that physiological GSV trafficking depends in part on the presence of IRAP in these vesicles, and that this process is regulated by tankyrase and probably its PARP activity.

Original languageEnglish (US)
Pages (from-to)279-290
Number of pages12
JournalBiochemical Journal
Volume402
Issue number2
DOIs
StatePublished - Mar 1 2007

Fingerprint

Tankyrases
Exocytosis
Insulin
Adipocytes
Glucose Transporter Type 4
Aminopeptidases
Phosphorylation
Facilitative Glucose Transport Proteins
Poly(ADP-ribose) Polymerases
leucyl-cystinyl aminopeptidase
Cell membranes
Adenosine Diphosphate
Muscle Cells
Small Interfering RNA
Carrier Proteins
Proteins
Cell Membrane

Keywords

  • Adipocytes
  • GLUT1
  • GLUT4
  • Insulin-responsive aminopeptidase (IRAP)
  • Iodixanol gradients
  • Tankyrase

ASJC Scopus subject areas

  • Biochemistry
  • Medicine(all)

Cite this

Insulin-stimulated exocytosis of GLUT4 is enhanced by IRAP and its partner tankyrase. / Yeh, Tsung Yin J; Sbodio, Juan; Tsun, Zhi Yang; Luo, Biao; Chi, Nai Wen.

In: Biochemical Journal, Vol. 402, No. 2, 01.03.2007, p. 279-290.

Research output: Contribution to journalArticle

Yeh, Tsung Yin J ; Sbodio, Juan ; Tsun, Zhi Yang ; Luo, Biao ; Chi, Nai Wen. / Insulin-stimulated exocytosis of GLUT4 is enhanced by IRAP and its partner tankyrase. In: Biochemical Journal. 2007 ; Vol. 402, No. 2. pp. 279-290.
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