Instabilotyping reveals unique mutational spectra in microsatellite-unstable gastric cancers

Yuriko Mori; Fumiaki Sato; Florin M. Selaru; Andreea Olaru; Kellie Perry; Martha C. Kimos; Gen Tamura; Nagahide Matsubara; Suna Wang; Yan Xu; Jing Yin; Tong Tong Zou; Barbara Leggett; Joanne Young; Toshihiro Nukiwa; O. Colin Stine; John M. Abraham; David Shibata; Stephen J. Meltzer

Instabilotyping reveals unique mutational spectra in microsatellite-unstable gastric cancers

Yuriko Mori, Fumiaki Sato, Florin M. Selaru, Andreea Olaru, Kellie Perry, Martha C. Kimos, Gen Tamura, Nagahide Matsubara, Suna Wang, Yan Xu, Jing Yin, Tong Tong Zou, Barbara Leggett, Joanne Young, Toshihiro Nukiwa, O. Colin Stine, John M. Abraham, David Shibata, Stephen J. Meltzer

Research output: Contribution to journal › Article › peer-review

88 Scopus citations

Abstract

Microsatellite instability (MSI) within coding regions causes frameshift mutations (FSMs). This type of mutation may inactivate tumor suppressor genes in cancers with frequent MSI (MSI-H cancers). To identify novel FSMs in gastric carcinogenesis in an unbiased and comprehensive manner, we screened for this type of mutation at 154 coding region repeat loci in 18 MSI-H gastric cancers. We also compared FSM rates and spectra in MSI-H gastric versus colorectal cancers. Thirteen novel loci showed FSMs in >20% of gastric tumors. Novel loci with the highest mutation frequencies included the activin type 2 receptor gene (44.4%), DKFZp564K112 (a homologue of the Drosophila tumor suppressor gene multi-sex-combs; 41.2%), and an endoplasmic reticulum chaperone protein gene SEC63 (37.5%). The mutational spectra for genes with high mutation frequencies were also significantly different between MSI-H gastric and colorectal cancers.

Original language	English (US)
Pages (from-to)	3641-3645
Number of pages	5
Journal	Cancer Research
Volume	62
Issue number	13
State	Published - Jul 1 2002
Externally published	Yes

ASJC Scopus subject areas

Oncology
Cancer Research

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Mori, Y., Sato, F., Selaru, F. M., Olaru, A., Perry, K., Kimos, M. C., Tamura, G., Matsubara, N., Wang, S., Xu, Y., Yin, J., Zou, T. T., Leggett, B., Young, J., Nukiwa, T., Stine, O. C., Abraham, J. M., Shibata, D., & Meltzer, S. J. (2002). Instabilotyping reveals unique mutational spectra in microsatellite-unstable gastric cancers. Cancer Research, 62(13), 3641-3645.

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title = "Instabilotyping reveals unique mutational spectra in microsatellite-unstable gastric cancers",

abstract = "Microsatellite instability (MSI) within coding regions causes frameshift mutations (FSMs). This type of mutation may inactivate tumor suppressor genes in cancers with frequent MSI (MSI-H cancers). To identify novel FSMs in gastric carcinogenesis in an unbiased and comprehensive manner, we screened for this type of mutation at 154 coding region repeat loci in 18 MSI-H gastric cancers. We also compared FSM rates and spectra in MSI-H gastric versus colorectal cancers. Thirteen novel loci showed FSMs in >20% of gastric tumors. Novel loci with the highest mutation frequencies included the activin type 2 receptor gene (44.4%), DKFZp564K112 (a homologue of the Drosophila tumor suppressor gene multi-sex-combs; 41.2%), and an endoplasmic reticulum chaperone protein gene SEC63 (37.5%). The mutational spectra for genes with high mutation frequencies were also significantly different between MSI-H gastric and colorectal cancers.",

author = "Yuriko Mori and Fumiaki Sato and Selaru, {Florin M.} and Andreea Olaru and Kellie Perry and Kimos, {Martha C.} and Gen Tamura and Nagahide Matsubara and Suna Wang and Yan Xu and Jing Yin and Zou, {Tong Tong} and Barbara Leggett and Joanne Young and Toshihiro Nukiwa and Stine, {O. Colin} and Abraham, {John M.} and David Shibata and Meltzer, {Stephen J.}",

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language = "English (US)",

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T1 - Instabilotyping reveals unique mutational spectra in microsatellite-unstable gastric cancers

AU - Mori, Yuriko

AU - Sato, Fumiaki

AU - Selaru, Florin M.

AU - Olaru, Andreea

AU - Perry, Kellie

AU - Kimos, Martha C.

AU - Tamura, Gen

AU - Matsubara, Nagahide

AU - Wang, Suna

AU - Xu, Yan

AU - Yin, Jing

AU - Zou, Tong Tong

AU - Leggett, Barbara

AU - Young, Joanne

AU - Nukiwa, Toshihiro

AU - Stine, O. Colin

AU - Abraham, John M.

AU - Shibata, David

AU - Meltzer, Stephen J.

PY - 2002/7/1

Y1 - 2002/7/1

N2 - Microsatellite instability (MSI) within coding regions causes frameshift mutations (FSMs). This type of mutation may inactivate tumor suppressor genes in cancers with frequent MSI (MSI-H cancers). To identify novel FSMs in gastric carcinogenesis in an unbiased and comprehensive manner, we screened for this type of mutation at 154 coding region repeat loci in 18 MSI-H gastric cancers. We also compared FSM rates and spectra in MSI-H gastric versus colorectal cancers. Thirteen novel loci showed FSMs in >20% of gastric tumors. Novel loci with the highest mutation frequencies included the activin type 2 receptor gene (44.4%), DKFZp564K112 (a homologue of the Drosophila tumor suppressor gene multi-sex-combs; 41.2%), and an endoplasmic reticulum chaperone protein gene SEC63 (37.5%). The mutational spectra for genes with high mutation frequencies were also significantly different between MSI-H gastric and colorectal cancers.

AB - Microsatellite instability (MSI) within coding regions causes frameshift mutations (FSMs). This type of mutation may inactivate tumor suppressor genes in cancers with frequent MSI (MSI-H cancers). To identify novel FSMs in gastric carcinogenesis in an unbiased and comprehensive manner, we screened for this type of mutation at 154 coding region repeat loci in 18 MSI-H gastric cancers. We also compared FSM rates and spectra in MSI-H gastric versus colorectal cancers. Thirteen novel loci showed FSMs in >20% of gastric tumors. Novel loci with the highest mutation frequencies included the activin type 2 receptor gene (44.4%), DKFZp564K112 (a homologue of the Drosophila tumor suppressor gene multi-sex-combs; 41.2%), and an endoplasmic reticulum chaperone protein gene SEC63 (37.5%). The mutational spectra for genes with high mutation frequencies were also significantly different between MSI-H gastric and colorectal cancers.

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Instabilotyping reveals unique mutational spectra in microsatellite-unstable gastric cancers

Abstract

ASJC Scopus subject areas

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