Abstract
Somatic mutation occurs frequently in rearranged and expressed immunoglobulin variable region genes in vivo. In contrast, V region hypermutation seldom occurs in antibody-forming cells in culture. The S107 mouse myeloma cell line is one of the few cell lines that has been observed to generate V region mutations frequently and spontaneously in vitro. Detailed examination reveals that both the S107 tumor and the cell line derived from it contain and express a duplicated heavy-chain gene. In culture, only one of the two heavy-chain genes undergoes both V and C region mutation, and variants with complex phenotypes and genotypes arise as a result of mutation and segregation of these duplicated genes.
Original language | English (US) |
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Pages (from-to) | 259-276 |
Number of pages | 18 |
Journal | Somatic Cell and Molecular Genetics |
Volume | 17 |
Issue number | 3 |
DOIs | |
State | Published - May 1991 |
Externally published | Yes |
ASJC Scopus subject areas
- Genetics
- Cell Biology