Insights into the physiology of Methylotenera mobilis as revealed by metagenome-based shotgun proteomic analysis

Gundula Bosch, Tiansong Wang, Ekaterina Latypova, Marina G. Kalyuzhnaya, Murray Hackett, Ludmila Chistoserdova

Research output: Contribution to journalArticle

Abstract

While the shotgun proteomics approach is gaining momentum in understanding microbial physiology, it remains limited by the paucity of high-quality genomic data, especially when it comes to poorly characterized newly identified phyla. At the same time, large-scale metagenomic sequencing projects produce datasets representing genomes of a variety of environmental microbes, although with lower sequence coverage and sequence quality. In this work we tested the utility of a metagenomic dataset enriched in sequences of environmental strains of Methylotenera mobilis, to assess the protein profile of a laboratory-cultivated strain, M. mobilis JLW8, as a proof of principle. We demonstrate that a large portion of the proteome predicted from the metagenomic sequence (approx. 20 %) could be identified with high confidence (three or more peptide sequences), thus gaining insights into the physiology of this bacterium, which represents a new genus within the family Methylophilaceae.

Original languageEnglish (US)
Pages (from-to)1103-1110
Number of pages8
JournalMicrobiology
Volume155
Issue number4
DOIs
StatePublished - Aug 10 2009
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology

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