Insights into nonspecific binding of homeodomains from a structure of MATα2 bound to DNA

Jun Aishima; Cynthia Wolberger

doi:10.1002/prot.10375

Insights into nonspecific binding of homeodomains from a structure of MATα2 bound to DNA

Jun Aishima, Cynthia Wolberger

School of Medicine

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

The 2.1-Å resolution crystal structure of the MATα2 homeodomain bound to DNA reveals the unexpected presence of two nonspecifically bound α2 homeodomains, in addition to the two α2 homeodomains bound to canonical α2 binding sites. One of the extra homeodomains makes few base-specific contacts, while the other extra homeodomain binds to DNA in a previously unobserved manner. This unusually bound homeodomain is rotated on the DNA, making possible major groove contacts by side-chains that normally do not contact the DNA. This alternate docking may represent one way in which homeodomains sample nonspecific DNA sequences.

Original language	English (US)
Pages (from-to)	544-551
Number of pages	8
Journal	Proteins: Structure, Function and Genetics
Volume	51
Issue number	4
DOIs	https://doi.org/10.1002/prot.10375
State	Published - Jun 1 2003

Keywords

DNA-binding protein
Homeodomain
Nonspecific binding
X-ray crystallography

ASJC Scopus subject areas

Structural Biology
Biochemistry
Molecular Biology

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10.1002/prot.10375

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title = "Insights into nonspecific binding of homeodomains from a structure of MATα2 bound to DNA",

abstract = "The 2.1-{\AA} resolution crystal structure of the MATα2 homeodomain bound to DNA reveals the unexpected presence of two nonspecifically bound α2 homeodomains, in addition to the two α2 homeodomains bound to canonical α2 binding sites. One of the extra homeodomains makes few base-specific contacts, while the other extra homeodomain binds to DNA in a previously unobserved manner. This unusually bound homeodomain is rotated on the DNA, making possible major groove contacts by side-chains that normally do not contact the DNA. This alternate docking may represent one way in which homeodomains sample nonspecific DNA sequences.",

keywords = "DNA-binding protein, Homeodomain, Nonspecific binding, X-ray crystallography",

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T1 - Insights into nonspecific binding of homeodomains from a structure of MATα2 bound to DNA

AU - Aishima, Jun

AU - Wolberger, Cynthia

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N2 - The 2.1-Å resolution crystal structure of the MATα2 homeodomain bound to DNA reveals the unexpected presence of two nonspecifically bound α2 homeodomains, in addition to the two α2 homeodomains bound to canonical α2 binding sites. One of the extra homeodomains makes few base-specific contacts, while the other extra homeodomain binds to DNA in a previously unobserved manner. This unusually bound homeodomain is rotated on the DNA, making possible major groove contacts by side-chains that normally do not contact the DNA. This alternate docking may represent one way in which homeodomains sample nonspecific DNA sequences.

AB - The 2.1-Å resolution crystal structure of the MATα2 homeodomain bound to DNA reveals the unexpected presence of two nonspecifically bound α2 homeodomains, in addition to the two α2 homeodomains bound to canonical α2 binding sites. One of the extra homeodomains makes few base-specific contacts, while the other extra homeodomain binds to DNA in a previously unobserved manner. This unusually bound homeodomain is rotated on the DNA, making possible major groove contacts by side-chains that normally do not contact the DNA. This alternate docking may represent one way in which homeodomains sample nonspecific DNA sequences.

KW - DNA-binding protein

KW - Homeodomain

KW - Nonspecific binding

KW - X-ray crystallography

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JO - Proteins: Structure, Function and Genetics

JF - Proteins: Structure, Function and Genetics

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ER -

Insights into nonspecific binding of homeodomains from a structure of MATα2 bound to DNA

Abstract

Keywords

ASJC Scopus subject areas

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