Insights into nonspecific binding of homeodomains from a structure of MATα2 bound to DNA

Jun Aishima, Cynthia Wolberger

Research output: Contribution to journalArticle

Abstract

The 2.1-Å resolution crystal structure of the MATα2 homeodomain bound to DNA reveals the unexpected presence of two nonspecifically bound α2 homeodomains, in addition to the two α2 homeodomains bound to canonical α2 binding sites. One of the extra homeodomains makes few base-specific contacts, while the other extra homeodomain binds to DNA in a previously unobserved manner. This unusually bound homeodomain is rotated on the DNA, making possible major groove contacts by side-chains that normally do not contact the DNA. This alternate docking may represent one way in which homeodomains sample nonspecific DNA sequences.

Original languageEnglish (US)
Pages (from-to)544-551
Number of pages8
JournalProteins: Structure, Function and Genetics
Volume51
Issue number4
DOIs
StatePublished - Jun 1 2003

Keywords

  • DNA-binding protein
  • Homeodomain
  • Nonspecific binding
  • X-ray crystallography

ASJC Scopus subject areas

  • Structural Biology
  • Biochemistry
  • Molecular Biology

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