Insights from gene arrays on the development and growth regulation of uterine leiomyomata

John C.M. Tsibris, James Segars, Domenico Coppola, Shrikant Mane, George D. Wilbanks, William F. O'Brien, William N. Spellacy

Research output: Contribution to journalArticlepeer-review

Abstract

Objective: To use microarray analysis as an unbiased approach to identify genes involved in the induction and growth of uterine leiomyomata. Design: Screen by arrays for up to 12,000 genes in leiomyoma (L) and control myometrium (M) from nine patients. Setting: University research laboratories. Patient(s): Nine patients in the follicular and luteal phases of the menstrual cycle. Intervention(s): mRNA from L and M was converted to biotin-labeled cRNA and hybridized to cDNA oligonucleotide sequences on the arrays. Main Outcome Measure(s): Greater than two-fold change in gene expression between leiomyoma and matched myometrium. Result(s): Prominent among the 67 genes overexpressed in L relative to M were dlk or Pref-1, doublecortin, JM27, ionotropic glutamate receptor subunit 2, apolipoprotein E3, IGF2, semaphorin F, myelin proteolipid protein, MEST, frizzled, CRABP II, stromelysin-3, and TGFβ3. The genes dlk, IGF2, and MEST are paternally expressed imprinted genes, and the others are involved in tissue differentiation and growth. Prominent among the 78 genes down-regulated in L relative to M were alcohol dehydrogenases 1α-γ, tryptase, dermatopontin, thrombospondin, coxsackievirus receptor, nur77, and c-kit. Conclusion(s): Arrays offer large-scale screening of mRNA expression, which will help us differentiate between the genes and metabolic pathways necessary for leiomyoma growth and those regulating myometrial contractions.

Original languageEnglish (US)
Pages (from-to)114-121
Number of pages8
JournalFertility and sterility
Volume78
Issue number1
DOIs
StatePublished - 2002

Keywords

  • Genomic imprinting
  • Mast cells
  • Myometrium
  • PPARγ
  • RNA editing
  • RXRα
  • Retinoic acid
  • Stem cell factor
  • c-kit
  • dlk

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynecology

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