Insights from gene arrays on the development and growth regulation of uterine leiomyomata

J. C M Tsibris, James Segars, Domenico Coppola, Shrikant Mane, George D. Wilbanks, William F. O'Brien, William N. Spellacy

Research output: Contribution to journalArticle

Abstract

Objective: To use microarray analysis as an unbiased approach to identify genes involved in the induction and growth of uterine leiomyomata. Design: Screen by arrays for up to 12,000 genes in leiomyoma (L) and control myometrium (M) from nine patients. Setting: University research laboratories. Patient(s): Nine patients in the follicular and luteal phases of the menstrual cycle. Intervention(s): mRNA from L and M was converted to biotin-labeled cRNA and hybridized to cDNA oligonucleotide sequences on the arrays. Main Outcome Measure(s): Greater than two-fold change in gene expression between leiomyoma and matched myometrium. Result(s): Prominent among the 67 genes overexpressed in L relative to M were dlk or Pref-1, doublecortin, JM27, ionotropic glutamate receptor subunit 2, apolipoprotein E3, IGF2, semaphorin F, myelin proteolipid protein, MEST, frizzled, CRABP II, stromelysin-3, and TGFβ3. The genes dlk, IGF2, and MEST are paternally expressed imprinted genes, and the others are involved in tissue differentiation and growth. Prominent among the 78 genes down-regulated in L relative to M were alcohol dehydrogenases 1α-γ, tryptase, dermatopontin, thrombospondin, coxsackievirus receptor, nur77, and c-kit. Conclusion(s): Arrays offer large-scale screening of mRNA expression, which will help us differentiate between the genes and metabolic pathways necessary for leiomyoma growth and those regulating myometrial contractions.

Original languageEnglish (US)
Pages (from-to)114-121
Number of pages8
JournalFertility and Sterility
Volume78
Issue number1
DOIs
StatePublished - 2002
Externally publishedYes

Fingerprint

Leiomyoma
Growth and Development
Genes
Myometrium
Matrix Metalloproteinase 11
Myelin Proteolipid Protein
Growth
Apolipoprotein E3
Semaphorins
CD36 Antigens
Ionotropic Glutamate Receptors
Tryptases
Uterine Contraction
Complementary RNA
Messenger RNA
Follicular Phase
Enterovirus
Alcohol Dehydrogenase
Luteal Phase
Microarray Analysis

Keywords

  • c-kit
  • dlk
  • Genomic imprinting
  • Mast cells
  • Myometrium
  • PPARγ
  • Retinoic acid
  • RNA editing
  • RXRα
  • Stem cell factor

ASJC Scopus subject areas

  • Obstetrics and Gynecology

Cite this

Tsibris, J. C. M., Segars, J., Coppola, D., Mane, S., Wilbanks, G. D., O'Brien, W. F., & Spellacy, W. N. (2002). Insights from gene arrays on the development and growth regulation of uterine leiomyomata. Fertility and Sterility, 78(1), 114-121. https://doi.org/10.1016/S0015-0282(02)03191-6

Insights from gene arrays on the development and growth regulation of uterine leiomyomata. / Tsibris, J. C M; Segars, James; Coppola, Domenico; Mane, Shrikant; Wilbanks, George D.; O'Brien, William F.; Spellacy, William N.

In: Fertility and Sterility, Vol. 78, No. 1, 2002, p. 114-121.

Research output: Contribution to journalArticle

Tsibris, JCM, Segars, J, Coppola, D, Mane, S, Wilbanks, GD, O'Brien, WF & Spellacy, WN 2002, 'Insights from gene arrays on the development and growth regulation of uterine leiomyomata', Fertility and Sterility, vol. 78, no. 1, pp. 114-121. https://doi.org/10.1016/S0015-0282(02)03191-6
Tsibris, J. C M ; Segars, James ; Coppola, Domenico ; Mane, Shrikant ; Wilbanks, George D. ; O'Brien, William F. ; Spellacy, William N. / Insights from gene arrays on the development and growth regulation of uterine leiomyomata. In: Fertility and Sterility. 2002 ; Vol. 78, No. 1. pp. 114-121.
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