Insight into the salivary transcriptome and proteome of dipetalogaster maxima

Teresa C.F. Assumpção, Sébastien Charneau, Paula B.M. Santiago, Ivo M.B. Francischetti, Zhaojing Meng, Carla N. Araújo, Van M. Pham, Rayner M.L. Queiroz, Cleudson Nery De Castro, Carlos André Ricart, Jaime M. Santana, Jose M.C. Ribeiro

Research output: Contribution to journalArticlepeer-review

Abstract

Dipetalogaster maxima is a blood-sucking Hemiptera that inhabits sylvatic areas in Mexico. It usually takes its blood meal from lizards, but following human population growth, it invaded suburban areas, feeding also on humans and domestic animals. Hematophagous insect salivary glands produce potent pharmacologic compounds that counteract host hemostasis, including anticlotting, antiplatelet, and vasodilatory molecules. To obtain further insight into the salivary biochemical and pharmacologic complexity of this insect, a cDNA library from its salivary glands was randomly sequenced. Salivary proteins were also submitted to one-and two-dimensional gel electrophoresis (1DE and 2DE) followed by mass spectrometry analysis. We present the analysis of a set of 2728 cDNA sequences, 1375 of which coded for proteins of a putative secretory nature. The saliva 2DE proteome displayed approximately 150 spots. The mass spectrometry analysis revealed mainly lipocalins, pallidipins, antigen 5-like proteins, and apyrases. The redundancy of sequence identification of saliva-secreted proteins suggests that proteins are present in multiple isoforms or derive from gene duplications.

Original languageEnglish (US)
Pages (from-to)669-679
Number of pages11
JournalJournal of proteome research
Volume10
Issue number2
DOIs
StatePublished - Feb 4 2011
Externally publishedYes

Keywords

  • D. maxima
  • Hematophagy
  • Proteome
  • Saliva
  • Transcriptome
  • Triatomine

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)

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