Insertion of N regions into heavy-chain genes is correlated with expression of terminal deoxytransferase in B cells

Stephen Desiderio, George D. Yancopoulos, Michael Paskind, Elise Thomas, Michael A. Boss, Nathaniel Landau, Frederick W. Alt, David Baltimore

Research output: Contribution to journalArticle

Abstract

The variable regions of immunoglobulin heavy chains are encoded in the germ line by three discrete DNA segments: VH (variable) elements, D (diversity) elements and JH (joining) elements. During the differentiation of B lymphocytes, individual segments from each group are brought together by recombination to form the complete VHDJ H variable region1-8. To understand these processes better, we have now isolated and sequenced molecular clones representing intermediates (DJH fusions) and final products ( V H-to-DJH joins) of heavy-chain gene rearrangement in two cell lines9,10 that represent analogues of cells at early stages of B-lymphocyte differentiation11-13. Heavy-chain gene assembly in one cell line but not in the other is accompanied by the appearance of short nucleotide insertions at the recombinational junctions. The generation of such insertions is positively correlated with the expression of terminal deoxynucleotidyl transferase in these lines.

Original languageEnglish (US)
Pages (from-to)752-755
Number of pages4
JournalNature
Volume311
Issue number5988
DOIs
StatePublished - 1984
Externally publishedYes

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B-Lymphocytes
Immunoglobulin Heavy Chains
DNA Nucleotidylexotransferase
Gene Rearrangement
Germ Cells
Genetic Recombination
Genes
Nucleotides
Clone Cells
Cell Line
DNA

ASJC Scopus subject areas

  • General

Cite this

Desiderio, S., Yancopoulos, G. D., Paskind, M., Thomas, E., Boss, M. A., Landau, N., ... Baltimore, D. (1984). Insertion of N regions into heavy-chain genes is correlated with expression of terminal deoxytransferase in B cells. Nature, 311(5988), 752-755. https://doi.org/10.1038/311752a0

Insertion of N regions into heavy-chain genes is correlated with expression of terminal deoxytransferase in B cells. / Desiderio, Stephen; Yancopoulos, George D.; Paskind, Michael; Thomas, Elise; Boss, Michael A.; Landau, Nathaniel; Alt, Frederick W.; Baltimore, David.

In: Nature, Vol. 311, No. 5988, 1984, p. 752-755.

Research output: Contribution to journalArticle

Desiderio, S, Yancopoulos, GD, Paskind, M, Thomas, E, Boss, MA, Landau, N, Alt, FW & Baltimore, D 1984, 'Insertion of N regions into heavy-chain genes is correlated with expression of terminal deoxytransferase in B cells', Nature, vol. 311, no. 5988, pp. 752-755. https://doi.org/10.1038/311752a0
Desiderio, Stephen ; Yancopoulos, George D. ; Paskind, Michael ; Thomas, Elise ; Boss, Michael A. ; Landau, Nathaniel ; Alt, Frederick W. ; Baltimore, David. / Insertion of N regions into heavy-chain genes is correlated with expression of terminal deoxytransferase in B cells. In: Nature. 1984 ; Vol. 311, No. 5988. pp. 752-755.
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