Inositol trisphosphate and thapsigargin discriminate endoplasmic reticulum stores of calcium in rat brain

Ajay Verma, David J. Hirsch, Michael R. Hanley, Ole Thastrup, S. Brogger Christensen, Solomon H Snyder

Research output: Contribution to journalArticle

Abstract

ATP dependent Ca2+ accumulation into oxalate-loaded rat brain microsomes is potently inhibited by thapsigargin with an IC50 of 2 nM and maximal inhibition at 10 nM. Approximately 15% of the total A23187-releasable microsomal calcium store is insensitive to thapsigargin concentrations up to 100 μM. Inositol-1,4,5-trisphosphate (IP3) maximally inhibits 40% of the net Ca2+ accumulation by whole brain microsomes. Its effects are non-additive with thapsigargin suggesting that the IP3-sensitive Ca2+ pool is a subset of the thapsigargin sensitive Ca2+ pool. Marked regional differences occur in Ca2+ transport rates and sensitivity to both thapsigargin and IP3.

Original languageEnglish (US)
Pages (from-to)811-816
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume172
Issue number2
DOIs
StatePublished - Oct 30 1990

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Thapsigargin
Inositol
Endoplasmic Reticulum
Rats
Brain
Calcium
Microsomes
Inositol 1,4,5-Trisphosphate
Oxalates
Calcimycin
Inhibitory Concentration 50
Adenosine Triphosphate

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Inositol trisphosphate and thapsigargin discriminate endoplasmic reticulum stores of calcium in rat brain. / Verma, Ajay; Hirsch, David J.; Hanley, Michael R.; Thastrup, Ole; Christensen, S. Brogger; Snyder, Solomon H.

In: Biochemical and Biophysical Research Communications, Vol. 172, No. 2, 30.10.1990, p. 811-816.

Research output: Contribution to journalArticle

Verma, Ajay ; Hirsch, David J. ; Hanley, Michael R. ; Thastrup, Ole ; Christensen, S. Brogger ; Snyder, Solomon H. / Inositol trisphosphate and thapsigargin discriminate endoplasmic reticulum stores of calcium in rat brain. In: Biochemical and Biophysical Research Communications. 1990 ; Vol. 172, No. 2. pp. 811-816.
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