Inositol trisphosphate and thapsigargin discriminate endoplasmic reticulum stores of calcium in rat brain

Ajay Verma, David J. Hirsch, Michael R. Hanley, Ole Thastrup, S. Brogger Christensen, Solomon H. Snyder

Research output: Contribution to journalArticlepeer-review

Abstract

ATP dependent Ca2+ accumulation into oxalate-loaded rat brain microsomes is potently inhibited by thapsigargin with an IC50 of 2 nM and maximal inhibition at 10 nM. Approximately 15% of the total A23187-releasable microsomal calcium store is insensitive to thapsigargin concentrations up to 100 μM. Inositol-1,4,5-trisphosphate (IP3) maximally inhibits 40% of the net Ca2+ accumulation by whole brain microsomes. Its effects are non-additive with thapsigargin suggesting that the IP3-sensitive Ca2+ pool is a subset of the thapsigargin sensitive Ca2+ pool. Marked regional differences occur in Ca2+ transport rates and sensitivity to both thapsigargin and IP3.

Original languageEnglish (US)
Pages (from-to)811-816
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume172
Issue number2
DOIs
StatePublished - Oct 30 1990

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Inositol trisphosphate and thapsigargin discriminate endoplasmic reticulum stores of calcium in rat brain'. Together they form a unique fingerprint.

Cite this