TY - JOUR
T1 - Inositol 1,4,5-trisphosphate receptor binding
T2 - Autoradiographic localization in rat brain
AU - Worley, P. F.
AU - Baraban, J. M.
AU - Snyder, S. H.
PY - 1989
Y1 - 1989
N2 - Inositol 1,4,5-trisphosphate is a second messenger generated by stimulation of the phosphoinositide cycle, thought to release calcium from intracellular stores. We have mapped the distribution of 3H-inositol 1,4,5-trisphosphate receptor binding sites in rat brain by autoradiographic techniques. The cerebellum contains the highest level of inositol 1,4,5-trisphosphate binding sites in brain, which appear to be selectively localized to Purkinje cells. Moderate levels of binding sites are present in the hippocampus, cerebral cortex, caudate, and substantia nigra. Lesion studies indicate that binding in the hippocampus is restricted to intrinsic neuronal elements and in the nigra is found on terminals of the striatonigral projection. Overall, the autoradiographic distribution of inositol 1,4,5-trisphosphate receptors resembles the distribution of phorbol ester binding sites associated with protein kinase C. However, the inositol 1,4,5-trisphosphate receptor has a more restricted distribution since it is not detectable in the spinal cord or olfactory bulb, regions with substantial levels of protein kinase C.
AB - Inositol 1,4,5-trisphosphate is a second messenger generated by stimulation of the phosphoinositide cycle, thought to release calcium from intracellular stores. We have mapped the distribution of 3H-inositol 1,4,5-trisphosphate receptor binding sites in rat brain by autoradiographic techniques. The cerebellum contains the highest level of inositol 1,4,5-trisphosphate binding sites in brain, which appear to be selectively localized to Purkinje cells. Moderate levels of binding sites are present in the hippocampus, cerebral cortex, caudate, and substantia nigra. Lesion studies indicate that binding in the hippocampus is restricted to intrinsic neuronal elements and in the nigra is found on terminals of the striatonigral projection. Overall, the autoradiographic distribution of inositol 1,4,5-trisphosphate receptors resembles the distribution of phorbol ester binding sites associated with protein kinase C. However, the inositol 1,4,5-trisphosphate receptor has a more restricted distribution since it is not detectable in the spinal cord or olfactory bulb, regions with substantial levels of protein kinase C.
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U2 - 10.1523/jneurosci.09-01-00339.1989
DO - 10.1523/jneurosci.09-01-00339.1989
M3 - Article
C2 - 2536419
AN - SCOPUS:0024500094
VL - 9
SP - 339
EP - 346
JO - Journal of Neuroscience
JF - Journal of Neuroscience
SN - 0270-6474
IS - 1
ER -