Inositol 1,4,5-Trisphosphate 3-Kinase A Associates with F-actin and Dendritic Spines via its N Terminus

Michael J. Schell, Christophe Erneux, Robin F. Irvine

Research output: Contribution to journalArticlepeer-review

98 Scopus citations


The consequences of the rapid 3-phosphorylation of inositol 1,4,5-trisphosphate (IP3) to produce inositol 1,3,4,5-tetrakisphosphate (IP4) via the action of IP3 3-kinases involve the control of calcium signals. Using green fluorescent protein constructs of full-length and truncated IP3 3-kinase isoform A expressed in HeLa cells, COS-7 cells, and primary neuronal cultures, we have defined a novel N-terminal 66-amino acid F-actin-binding region that localizes the kinase to dendritic spines. The region is necessary and sufficient for binding F-actin and consists of a proline-rich stretch followed by a predicted α-helix. We also localized endogenous IP3 3-kinase A to the dendritic spines of pyramidal neurons in primary hippocampal cultures, where it is co-localized postsynaptically with calcium/calmodulin-dependent protein kinase II. Our experiments suggest a link between inositol phosphate metabolism, calcium signaling, and the actin cytoskeleton in dendritic spines. The phosphorylation of IP3 in dendritic spines to produce IP4 is likely to be important for modulating the compartmentalization of calcium at synapses.

Original languageEnglish (US)
Pages (from-to)37537-37546
Number of pages10
JournalJournal of Biological Chemistry
Issue number40
StatePublished - Oct 5 2001
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology


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