@article{3a8911ec8b9d4b8397b90d28f0e39865,
title = "Innate control of actin nucleation determines two distinct migration behaviours in dendritic cells",
abstract = "Dendritic cell (DC) migration in peripheral tissues serves two main functions: antigen sampling by immature DCs, and chemokine-guided migration towards lymphatic vessels (LVs) on maturation. These migratory events determine the efficiency of the adaptive immune response. Their regulation by the core cell locomotion machinery has not been determined. Here, we show that the migration of immature DCs depends on two main actin pools: a RhoA-mDia1-dependent actin pool located at their rear, which facilitates forward locomotion; and a Cdc42-Arp2/3-dependent actin pool present at their front, which limits migration but promotes antigen capture. Following TLR4-MyD88-induced maturation, Arp2/3-dependent actin enrichment at the cell front is markedly reduced. Consequently, mature DCs switch to a faster and more persistent mDia1-dependent locomotion mode that facilitates chemotactic migration to LVs and lymph nodes. Thus, the differential use of actin-nucleating machineries optimizes the migration of immature and mature DCs according to their specific function.",
author = "Pablo Vargas and Paolo Maiuri and Marine Bretou and Sa{\'e}z, {Pablo J.} and Paolo Pierobon and Mathieu Maurin and M{\'e}lanie Chabaud and Danielle Lankar and Dorian Obino and Emmanuel Terriac and Matthew Raab and Thiam, {Hawa Racine} and Thomas Brocker and Kitchen-Goosen, {Susan M.} and Alberts, {Arthur S.} and Praveen Sunareni and Sheng Xia and Rong Li and Raphael Voituriez and Matthieu Piel and Lennon-Dum{\'e}nil, {Ana Maria}",
note = "Funding Information: The authors thank the PICT IBiSA platform at Institut Curie (CNRS UMR144, especially V. Fraisier) and the Institut Curie animal facility. P.V. thanks B. Goic for support, patience and critical reading of the manuscript. P.V. was supported by fellowships from Region Ile-de-France, Fondation pour la Recherche M{\'e}dicale (FRM) and Institut Curie. M.B. and M.C. benefited from fellowships from Association pour la Recherche contre le Cancer and FRM respectively. S.M.K.-G. and A.S.A. were supported by the Grand Rapids Community and the Lunn Hope Foundations, the Van Andel Endowment, and the Purple Community. T.B. was supported by DFG SFB 1054-B03 and SFB 914-A06. This work was financially supported by grants from: the City of Paris, the European Research Council and the DCBIOL Labex (ANR-10-IDEX-0001-02-PSL∗ and ANR-11-LABX-0043) to A.-M.L.-D. (Strapacemi 243103) and the Association Nationale pour la Recherche (ANR-09-PIRI-0027-PCVI) and the InnaBiosant{\'e} foundation (Micemico) to A.-M.L.-D., M.P. and R.V.",
year = "2016",
month = jan,
day = "1",
doi = "10.1038/ncb3284",
language = "English (US)",
volume = "18",
pages = "43--53",
journal = "Nature cell biology",
issn = "1465-7392",
publisher = "Nature Publishing Group",
number = "1",
}