Injury-activated transforming growth factor β controls mobilization of mesenchymal stem cells for tissue remodeling

Mei Wan, Changjun Li, Gehua Zhen, Kai Jiao, Wenying He, Xiaofeng Jia, Weishan Wang, Chenhui Shi, Qiujuan Xing, Yiu Fai Chen, Suzanne M Jan De Beur, Bing Yu, Xu Cao

Research output: Contribution to journalArticle

Abstract

Upon secretion, transforming growth factor β (TGFβ) is maintained in a sequestered state in extracellular matrix as a latent form. The latent TGFβ is considered as a molecular sensor that releases active TGFβ in response to the perturbations of the extracellular matrix at the situations of mechanical stress, wound repair, tissue injury, and inflammation. The biological implication of the temporal discontinuity of TGFβ storage in the matrix and its activation is obscure. Here, using several animal models in which latent TGFβ is activated in vascular matrix in response to injury of arteries, we show that active TGFβ controls the mobilization and recruitment of mesenchymal stem cells (MSCs) to participate in tissue repair and remodeling. MSCs were mobilized into the peripheral blood in response to vascular injury and recruited to the injured sites where they gave rise to both endothelial cells for re-endothelialization and myofibroblastic cells to form thick neointima. TGFβs were activated in the vascular matrix in both rat and mouse models of mechanical injury of arteries. Importantly, the active TGFβ released from the injured vessels is essential to induce the migration of MSCs, and cascade expression of monocyte chemotactic protein-1 stimulated by TGFβ amplifies the signal for migration. Moreover, sustained high levels of active TGFβ were observed in peripheral blood, and at the same time points following injury, Sca1+CD29+CD1-b2CD45- MSCs, in which 91% are nestin+ cells, were mobilized to peripheral blood and recruited to the remodeling arteries. Intravenously injection of recombinant active TGFβ1 in uninjured mice rapidly mobilized MSCs into circulation. Furthermore, inhibitor of TGFβ type I receptor blocked the mobilization and recruitment of MSCs to the injured arteries. Thus, TGFβ is an injury-activated messenger essential for the mobilization and recruitment of MSCs to participate in tissue repair/remodeling.

Original languageEnglish (US)
Pages (from-to)2498-2511
Number of pages14
JournalStem Cells
Volume30
Issue number11
DOIs
StatePublished - Nov 2012

Fingerprint

Transforming Growth Factors
Mesenchymal Stromal Cells
Wounds and Injuries
Arteries
Extracellular Matrix
Blood Vessels
Neointima
Nestin
Mechanical Stress
Growth Factor Receptors
Chemokine CCL2
Vascular System Injuries
Endothelial Cells
Animal Models
Inflammation
Injections

Keywords

  • Cell mobilization
  • Mesenchymal stem cells
  • TGF β activation
  • Vascular remodeling

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Molecular Medicine

Cite this

Injury-activated transforming growth factor β controls mobilization of mesenchymal stem cells for tissue remodeling. / Wan, Mei; Li, Changjun; Zhen, Gehua; Jiao, Kai; He, Wenying; Jia, Xiaofeng; Wang, Weishan; Shi, Chenhui; Xing, Qiujuan; Chen, Yiu Fai; Jan De Beur, Suzanne M; Yu, Bing; Cao, Xu.

In: Stem Cells, Vol. 30, No. 11, 11.2012, p. 2498-2511.

Research output: Contribution to journalArticle

Wan, Mei ; Li, Changjun ; Zhen, Gehua ; Jiao, Kai ; He, Wenying ; Jia, Xiaofeng ; Wang, Weishan ; Shi, Chenhui ; Xing, Qiujuan ; Chen, Yiu Fai ; Jan De Beur, Suzanne M ; Yu, Bing ; Cao, Xu. / Injury-activated transforming growth factor β controls mobilization of mesenchymal stem cells for tissue remodeling. In: Stem Cells. 2012 ; Vol. 30, No. 11. pp. 2498-2511.
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AU - Wan, Mei

AU - Li, Changjun

AU - Zhen, Gehua

AU - Jiao, Kai

AU - He, Wenying

AU - Jia, Xiaofeng

AU - Wang, Weishan

AU - Shi, Chenhui

AU - Xing, Qiujuan

AU - Chen, Yiu Fai

AU - Jan De Beur, Suzanne M

AU - Yu, Bing

AU - Cao, Xu

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