Initiation of histone synthesis by f-met-tRNAf in an ascites cell-free system

M. Jacobs-Lorena, C. Baglioni

Research output: Contribution to journalArticlepeer-review


Formyl-[35S]methionine is incorporated into histones synthesized by a mouse ascites cell-free system supplemented with histone mRNA and f-[35S]met-tRNAf from yeast. Most of the [35S]methionine incorporated can be shown to be at the N-terminus by Edman degradation after deformylation. This indicates that methionine can be used as the initiator amino acid for histones in this cell-free system.

Original languageEnglish (US)
Pages (from-to)113-117
Number of pages5
JournalMolecular Biology Reports
Issue number2
StatePublished - Aug 1 1973
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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