Initiation of histone synthesis by f-met-tRNAf in an ascites cell-free system

M. Jacobs-Lorena; C. Baglioni

doi:10.1007/BF00357590

Initiation of histone synthesis by f-met-tRNA_f in an ascites cell-free system

M. Jacobs-Lorena, C. Baglioni

Research output: Contribution to journal › Article › peer-review

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Abstract

Formyl-[³⁵S]methionine is incorporated into histones synthesized by a mouse ascites cell-free system supplemented with histone mRNA and f-[³⁵S]met-tRNA_f from yeast. Most of the [³⁵S]methionine incorporated can be shown to be at the N-terminus by Edman degradation after deformylation. This indicates that methionine can be used as the initiator amino acid for histones in this cell-free system.

Original language	English (US)
Pages (from-to)	113-117
Number of pages	5
Journal	Molecular Biology Reports
Volume	1
Issue number	2
DOIs	https://doi.org/10.1007/BF00357590
State	Published - Aug 1973
Externally published	Yes

ASJC Scopus subject areas

Molecular Biology
Genetics

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10.1007/BF00357590

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title = "Initiation of histone synthesis by f-met-tRNAf in an ascites cell-free system",

abstract = "Formyl-[35S]methionine is incorporated into histones synthesized by a mouse ascites cell-free system supplemented with histone mRNA and f-[35S]met-tRNAf from yeast. Most of the [35S]methionine incorporated can be shown to be at the N-terminus by Edman degradation after deformylation. This indicates that methionine can be used as the initiator amino acid for histones in this cell-free system.",

author = "M. Jacobs-Lorena and C. Baglioni",

year = "1973",

month = aug,

doi = "10.1007/BF00357590",

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AB - Formyl-[35S]methionine is incorporated into histones synthesized by a mouse ascites cell-free system supplemented with histone mRNA and f-[35S]met-tRNAf from yeast. Most of the [35S]methionine incorporated can be shown to be at the N-terminus by Edman degradation after deformylation. This indicates that methionine can be used as the initiator amino acid for histones in this cell-free system.

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Initiation of histone synthesis by f-met-tRNA_f in an ascites cell-free system

Abstract

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