Inhibitory synaptic interactions between cochlear nuclei: Evidence from an in vitro whole brain study

Alexander L. Babalian, David Kay Ryugo, Mattheus W. Vischer, Eric M. Rouiller

Research output: Contribution to journalArticle

Abstract

Using guinea-pig isolated whole brain preparation in vitro, synaptic responses to electrical stimulation of auditory nerves were examined in intracellularly recorded and stained neurons of posteroventral and dorsal divisions of the cochlear nucleus. Stimulation of the contralateral auditory nerve evoked exclusively IPSPs in 70% of neurons, With amplitude of 2.3 ± 1.2 mV. Neurons of all major cell types were inhibited from the contralateral side. In the majority of responding cells (78%) IPSPs were induced at latencies of 39 ms suggesting di- and trisynaptic connections from contralateral auditory afferents or, respectively, mono-and disynaptic connections from the contralateral cochlear nucleus. Few cells responded with long-latency IPSPs (13.5-23 ms), indicating involvement of polysynaptic pathways. These data demonstrate the existence of functional, direct and indirect inhibitory connections between the cochlear nuclei.

Original languageEnglish (US)
Pages (from-to)1913-1917
Number of pages5
JournalNeuroReport
Volume10
Issue number9
StatePublished - Jun 23 1999

Fingerprint

Cochlear Nucleus
Inhibitory Postsynaptic Potentials
Cochlear Nerve
Neurons
Brain
Electric Stimulation
Guinea Pigs
In Vitro Techniques

Keywords

  • Cochlear nucleus
  • EPSP
  • Intracellular recording and staining
  • IPSP
  • Isolated whole brain
  • Synaptic potentials

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Inhibitory synaptic interactions between cochlear nuclei : Evidence from an in vitro whole brain study. / Babalian, Alexander L.; Ryugo, David Kay; Vischer, Mattheus W.; Rouiller, Eric M.

In: NeuroReport, Vol. 10, No. 9, 23.06.1999, p. 1913-1917.

Research output: Contribution to journalArticle

Babalian, AL, Ryugo, DK, Vischer, MW & Rouiller, EM 1999, 'Inhibitory synaptic interactions between cochlear nuclei: Evidence from an in vitro whole brain study', NeuroReport, vol. 10, no. 9, pp. 1913-1917.
Babalian, Alexander L. ; Ryugo, David Kay ; Vischer, Mattheus W. ; Rouiller, Eric M. / Inhibitory synaptic interactions between cochlear nuclei : Evidence from an in vitro whole brain study. In: NeuroReport. 1999 ; Vol. 10, No. 9. pp. 1913-1917.
@article{00d82660cdbb42639065bb6333a42410,
title = "Inhibitory synaptic interactions between cochlear nuclei: Evidence from an in vitro whole brain study",
abstract = "Using guinea-pig isolated whole brain preparation in vitro, synaptic responses to electrical stimulation of auditory nerves were examined in intracellularly recorded and stained neurons of posteroventral and dorsal divisions of the cochlear nucleus. Stimulation of the contralateral auditory nerve evoked exclusively IPSPs in 70{\%} of neurons, With amplitude of 2.3 ± 1.2 mV. Neurons of all major cell types were inhibited from the contralateral side. In the majority of responding cells (78{\%}) IPSPs were induced at latencies of 39 ms suggesting di- and trisynaptic connections from contralateral auditory afferents or, respectively, mono-and disynaptic connections from the contralateral cochlear nucleus. Few cells responded with long-latency IPSPs (13.5-23 ms), indicating involvement of polysynaptic pathways. These data demonstrate the existence of functional, direct and indirect inhibitory connections between the cochlear nuclei.",
keywords = "Cochlear nucleus, EPSP, Intracellular recording and staining, IPSP, Isolated whole brain, Synaptic potentials",
author = "Babalian, {Alexander L.} and Ryugo, {David Kay} and Vischer, {Mattheus W.} and Rouiller, {Eric M.}",
year = "1999",
month = "6",
day = "23",
language = "English (US)",
volume = "10",
pages = "1913--1917",
journal = "NeuroReport",
issn = "0959-4965",
publisher = "Lippincott Williams and Wilkins",
number = "9",

}

TY - JOUR

T1 - Inhibitory synaptic interactions between cochlear nuclei

T2 - Evidence from an in vitro whole brain study

AU - Babalian, Alexander L.

AU - Ryugo, David Kay

AU - Vischer, Mattheus W.

AU - Rouiller, Eric M.

PY - 1999/6/23

Y1 - 1999/6/23

N2 - Using guinea-pig isolated whole brain preparation in vitro, synaptic responses to electrical stimulation of auditory nerves were examined in intracellularly recorded and stained neurons of posteroventral and dorsal divisions of the cochlear nucleus. Stimulation of the contralateral auditory nerve evoked exclusively IPSPs in 70% of neurons, With amplitude of 2.3 ± 1.2 mV. Neurons of all major cell types were inhibited from the contralateral side. In the majority of responding cells (78%) IPSPs were induced at latencies of 39 ms suggesting di- and trisynaptic connections from contralateral auditory afferents or, respectively, mono-and disynaptic connections from the contralateral cochlear nucleus. Few cells responded with long-latency IPSPs (13.5-23 ms), indicating involvement of polysynaptic pathways. These data demonstrate the existence of functional, direct and indirect inhibitory connections between the cochlear nuclei.

AB - Using guinea-pig isolated whole brain preparation in vitro, synaptic responses to electrical stimulation of auditory nerves were examined in intracellularly recorded and stained neurons of posteroventral and dorsal divisions of the cochlear nucleus. Stimulation of the contralateral auditory nerve evoked exclusively IPSPs in 70% of neurons, With amplitude of 2.3 ± 1.2 mV. Neurons of all major cell types were inhibited from the contralateral side. In the majority of responding cells (78%) IPSPs were induced at latencies of 39 ms suggesting di- and trisynaptic connections from contralateral auditory afferents or, respectively, mono-and disynaptic connections from the contralateral cochlear nucleus. Few cells responded with long-latency IPSPs (13.5-23 ms), indicating involvement of polysynaptic pathways. These data demonstrate the existence of functional, direct and indirect inhibitory connections between the cochlear nuclei.

KW - Cochlear nucleus

KW - EPSP

KW - Intracellular recording and staining

KW - IPSP

KW - Isolated whole brain

KW - Synaptic potentials

UR - http://www.scopus.com/inward/record.url?scp=0033597578&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033597578&partnerID=8YFLogxK

M3 - Article

C2 - 10501532

AN - SCOPUS:0033597578

VL - 10

SP - 1913

EP - 1917

JO - NeuroReport

JF - NeuroReport

SN - 0959-4965

IS - 9

ER -