TY - JOUR
T1 - Inhibitory Synapses Are Repeatedly Assembled and Removed at Persistent Sites In Vivo
AU - Villa, Katherine L.
AU - Berry, Kalen P.
AU - Subramanian, Jaichandar
AU - Cha, Jae Won
AU - Oh, Won Chan
AU - Kwon, Hyung Bae
AU - Kubota, Yoshiyuki
AU - So, Peter T.C.
AU - Nedivi, Elly
N1 - Funding Information:
We thank Dr. Charles Jennings; Dr. Jeff Hoch; and members of the Nedivi lab, especially Dr. Sven Loebrich, for comments on the manuscript. We thank Dr. Nelson Spruston for helpful discussions. We also thank Ms. Sayuri Hatada (NIPS) for assistance with EM histology; Dr. Xin Man (Hitachi) for help with the FIB/SEM imaging; and Ms. Chihiro Shiozu, Ms. Hiroko Kita (NIPS), and Mr. Alsayed Abdelhamid Mohamed for work on the 3D reconstruction of the electron microscopy. This work was sponsored by National Eye Institute grant RO1 EY017656 and partly grant RO1 EY011894 to E.N.; NIH P41EB015871-26A1, 4R44EB012415-02, NSF CBET-0939511, the Singapore-MIT Alliance 2, and the Singapore-MIT Alliance for Science and Technology Center to J.W.C. and P.T.C.S.; and F31AG044061 to K.L.V. Partial support for K.L.V. and K.P.B. was provided by NIH Pre-Doctoral Training Grant T32GM007287. Y.K. was supported by grant-in-aid for scientific research from the Ministry of Education, Culture, Sports, Science and Technology of Japan (B) 25290012; on Innovative Areas-Adaptive circuit shift (Number 3603); 26112006 and 15H01456; and the Imaging Science Project of CNSI, National Institutes of Natural Sciences (NINS) IS261004. H.-B.K. was supported by R01 MH107460.
Funding Information:
We thank Dr. Charles Jennings; Dr. Jeff Hoch; and members of the Nedivi lab, especially Dr. Sven Loebrich, for comments on the manuscript. We thank Dr. Nelson Spruston for helpful discussions. We also thank Ms. Sayuri Hatada (NIPS) for assistance with EM histology; Dr. Xin Man (Hitachi) for help with the FIB/SEM imaging; and Ms. Chihiro Shiozu, Ms. Hiroko Kita (NIPS), and Mr. Alsayed Abdelhamid Mohamed for work on the 3D reconstruction of the electron microscopy. This work was sponsored by National Eye Institute grant RO1 EY017656 and partly grant RO1 EY011894 to E.N.; NIH P41EB015871-26A1, 4R44EB012415-02, NSF CBET-0939511, the Singapore-MIT Alliance 2, and the Singapore-MIT Alliance for Science and Technology Center to J.W.C. and P.T.C.S.; and F31AG044061 to K.L.V. Partial support for K.L.V. and K.P.B. was provided by NIH Pre-Doctoral Training Grant T32GM007287. Y.K. was supported by grant-in-aid for scientific research from the Ministry of Education, Culture, Sports, Science and Technology of Japan (B) 25290012; on Innovative Areas-Adaptive circuit shift (Number 3603); 26112006 and 15H01456; and the Imaging Science Project of CNSI, National Institutes of Natural Sciences (NINS) IS261004. H.-B.K. was supported by R01 MH107460.
Publisher Copyright:
© 2016 Elsevier Inc.
PY - 2016/2/17
Y1 - 2016/2/17
N2 - Older concepts of a hard-wired adult brain have been overturned in recent years by in vivo imaging studies revealing synaptic remodeling, now thought to mediate rearrangements in microcircuit connectivity. Using three-color labeling and spectrally resolved two-photon microscopy, we monitor in parallel the daily structural dynamics (assembly or removal) of excitatory and inhibitory postsynaptic sites on the same neurons in mouse visual cortex in vivo. We find that dynamic inhibitory synapses often disappear and reappear again in the same location. The starkest contrast between excitatory and inhibitory synapse dynamics is on dually innervated spines, where inhibitory synapses frequently recur while excitatory synapses are stable. Monocular deprivation, a model of sensory input-dependent plasticity, shortens inhibitory synapse lifetimes and lengthens intervals to recurrence, resulting in a new dynamic state with reduced inhibitory synaptic presence. Reversible structural dynamics indicate a fundamentally new role for inhibitory synaptic remodeling-flexible, input-specific modulation of stable excitatory connections. Synaptic remodeling observed in vivo is commonly thought to represent rearrangements in microcircuit connectivity. Villa et al. observe a new, reversible type of synapse dynamics, unique to inhibitory synapses, which could provide flexible, input-specific gating of stable excitatory connections.
AB - Older concepts of a hard-wired adult brain have been overturned in recent years by in vivo imaging studies revealing synaptic remodeling, now thought to mediate rearrangements in microcircuit connectivity. Using three-color labeling and spectrally resolved two-photon microscopy, we monitor in parallel the daily structural dynamics (assembly or removal) of excitatory and inhibitory postsynaptic sites on the same neurons in mouse visual cortex in vivo. We find that dynamic inhibitory synapses often disappear and reappear again in the same location. The starkest contrast between excitatory and inhibitory synapse dynamics is on dually innervated spines, where inhibitory synapses frequently recur while excitatory synapses are stable. Monocular deprivation, a model of sensory input-dependent plasticity, shortens inhibitory synapse lifetimes and lengthens intervals to recurrence, resulting in a new dynamic state with reduced inhibitory synaptic presence. Reversible structural dynamics indicate a fundamentally new role for inhibitory synaptic remodeling-flexible, input-specific modulation of stable excitatory connections. Synaptic remodeling observed in vivo is commonly thought to represent rearrangements in microcircuit connectivity. Villa et al. observe a new, reversible type of synapse dynamics, unique to inhibitory synapses, which could provide flexible, input-specific gating of stable excitatory connections.
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U2 - 10.1016/j.neuron.2016.01.010
DO - 10.1016/j.neuron.2016.01.010
M3 - Article
C2 - 26853302
AN - SCOPUS:84958121334
VL - 89
SP - 756
EP - 769
JO - Neuron
JF - Neuron
SN - 0896-6273
IS - 4
ER -