Inhibition of xanthine oxidase-xanthine-iron mediated lipid peroxidation by eugenol in liposomes

Nagababu Enika, N. Lakshmaiah

Research output: Contribution to journalArticle

Abstract

The effect of eugenol on xanthine oxidase (XO) xanthine(X)-Fe+3-ADP mediated lipid peroxidation was studied in liver microsomal lipid liposomes. Eugenol inhibited the lipid peroxidation in a dose dependent manner as assessed by formation of thiobarbituric acid reactive substances. When tested for its effect on XO activity per se, (by measuring uric acid formation) eugenol inhibited the enzyme to an extent of 85% at 10 μm concentration and hence formation of O.2 - also. However, the concentration of eugenol required for XO inhibition was more in presence of metal chelators such as EDTA, EGTA and DETAPAC, but not in presence of deferoxamine, ADP and citrate. The antiperoxidative effect of eugenol was about 35 times more and inhibition of XO was about 5 times higher as compared to the effect of allopurinol. Eugenol did not scavenge O.2 - generated by phenazine methosulfate and NAD but inhibited propagation of peroxidation catalyzed by Fe2+ EDTA and lipid hydroperoxide containing liposomes. Eugenol inhibits XO-X-Fe+3 ADP mediated peroxidation by inhibiting the XO activity per se in addition to quenching various radical species.

Original languageEnglish (US)
Pages (from-to)65-71
Number of pages7
JournalMolecular and Cellular Biochemistry
Volume166
Issue number1-2
DOIs
StatePublished - 1997
Externally publishedYes

Fingerprint

Eugenol
Xanthine
Xanthine Oxidase
Liposomes
Lipid Peroxidation
Iron
Lipids
Edetic Acid
Methylphenazonium Methosulfate
Pentetic Acid
Allopurinol
Deferoxamine
Thiobarbituric Acid Reactive Substances
Lipid Peroxides
Egtazic Acid
Chelating Agents
Uric Acid
Citric Acid
Liver
NAD

Keywords

  • Antioxidant
  • Eugenol
  • Free radical
  • Xanthine oxidase peroxidation

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Inhibition of xanthine oxidase-xanthine-iron mediated lipid peroxidation by eugenol in liposomes. / Enika, Nagababu; Lakshmaiah, N.

In: Molecular and Cellular Biochemistry, Vol. 166, No. 1-2, 1997, p. 65-71.

Research output: Contribution to journalArticle

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