Inhibition of tumor growth mediated by lymphocytes sensitized in vitro to a syngeneic murine teratocarcinoma 402AX

P. F. Bartlett, B. A. Fenderson, M. Edidin

Research output: Contribution to journalArticlepeer-review

Abstract

TerC, a cell line derived from a strain 129 teratocarcinoma 402AX, was used to sensitize syngeneic 129 (H-2bc) splenic lymphocytes in vitro. The effector cells generated inhibited in vitro growth of TerC as measured by an 125I-IUDR post-labeling technique. It was also shown, with a modified Winn assay, that the sensitized cells were effective in preventing TerC growth in vivo. The effector lymphocyte was nonadherent to nylon wool, was sensitive to anti-Thy-1 2 + C, and was phenotypically Ly 1-2+. The anti-TerC effector T lymphocytes were not functional in a 51Cr-release assay. However, this failure to lyse appears not to be due to some intrinsic membrane resistance since both BCG and ConA-activated killers were able to lyse TerC. The TerC-sensitized lymphocytes displayed no H-2 restriction and were able to growth inhibit in vitro a wide range of tumorigenic cell lines, e.g., P815 (H-2d), EL-4 (H-2b),Sal (H-2a), and BALB/c (H-2d) 3T12. Mouse blastocyst cell lines were also inhibited. BALB/c 3T3 and mouse fibroblast cell strains were not growth inhibited. Thus, it appears that oncofetal antigens expressed on TerC are capable of initiating a cell-mediated response and that these antigenic Specificities are shared by many transformed cell lines.

Original languageEnglish (US)
Pages (from-to)1211-1217
Number of pages7
JournalJournal of Immunology
Volume120
Issue number4
StatePublished - Dec 1 1978

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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