Inhibition of the development of N-nitrosomethylbenzylamine-induced esophageal tumors in rats by strawberries and aspirin, alone and in combination

Pan Pan, Daniel S. Peiffer, Yi Wen Huang, Kiyoko Oshima, Gary D. Stoner, Li Shu Wang

Research output: Contribution to journalArticlepeer-review

Abstract

BACKGROUND: Esophageal squamous cell carcinoma (ESCC) is one of two subtypes of esophageal cancer, with high incidence and mortality rates in developing countries. OBJECTIVE: The current study investigated the potential chemoprotective effects of strawberries and aspirin against the development of rat esophageal papillomas, the precursors to ESCC. METHODS: Using a prevention model, we administered study diets to rats before, during, and after N-nitrosomethylbenzylamine (NMBA) treatment. The effects of the four diets were evaluated: the control diet, 5% strawberry powder in the control diet, 0.01% aspirin in the drinking water, and the combination of strawberries and aspirin. At week 25, we euthanized all the rats and collected their esophagi to quantify tumor incidence, multiplicity, and burden, as well as for molecular analysis. RESULTS: Both strawberries and aspirin significantly decreased esophageal tumor multiplicity, with the combination causing the most robust suppression. Aspirin alone and the combination decreased the total tumor burden in the esophagus. None of the diets had a significant effect on tumor incidence or the expression of COX-1 and COX-2. Strawberries and aspirin, alone and in combination, significantly suppressed squamous epithelial cell proliferation (PCNA). CONCLUSIONS: Strawberries, aspirin, and their combination exhibit chemoprotective effects against NMBA-induced esophageal tumors in rats.

Original languageEnglish (US)
Pages (from-to)137-146
Number of pages10
JournalJournal of Berry Research
Volume8
Issue number2
DOIs
StatePublished - 2018

Keywords

  • Esophageal squamous cell carcinoma
  • PCNA
  • aspirin
  • strawberries

ASJC Scopus subject areas

  • Food Science
  • Biochemistry
  • Agronomy and Crop Science
  • Soil Science
  • Plant Science
  • Horticulture

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