Inhibition of superoxide generation from fMLP-stimulated leukocytes by high concentrations of nitric oxide or peroxynitrite: Characterization by electron spin resonance spectroscopy

Masaichi Chang il Lee, Hirofumi Shoji, Tomoko Komatsu, Fumihiko Yoshino, Yoichi Ohmori, Jay L. Zweier

Research output: Contribution to journalArticle

Abstract

This present study examined the effects of high concentrations of nitric oxide (NO*) and peroxynitrite (ONOO-) on superoxide (O2•-) production from formyl-methionyl-leucyl-phenylalanine (fMLP)-stimulated polymorphonuclear leukocytes (PMNs) by using electron spin resonance (ESR) and spin trapping with 5-(diethoxyphosphoryl)-5-methyl-1-pyrroline-N-oxide (DEPMPO). We demonstrated that ONOO- (100 μM) decreased the ESR signal of DEPMPO-OOH from fMLP-activated PMNs, indicating the inhibition of O2•- generation, while it enhanced the signal of DEPMPO-OH. Inhibition of the respiratory burst was also observed when PMNs were pre-exposed to high concentrations of NO* (100 μM), generated by the NO* donor NOR-1, 30 min prior to stimulation with fMLP. NOR-1 inhibited O2•- generation more effectively under conditions in which ONOO- was formed concurrently. The ability of high concentrations of either ONOO- or NO* to inhibit O2•- generation from fMLP-stimulated PMNs is relevant to pathophysiological conditions, such as severe inflammation, in which NO* or ONOO- production can be significantly elevated.

Original languageEnglish (US)
Pages (from-to)271-275
Number of pages5
JournalRedox Report
Volume7
Issue number5
DOIs
StatePublished - 2002

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methionyl-leucyl-phenylalanine
Electron spin resonance spectroscopy
Peroxynitrous Acid
Electron Spin Resonance Spectroscopy
Superoxides
Nitric Oxide
Leukocytes
Paramagnetic resonance
Spin Trapping
Respiratory Burst
Nitric Oxide Donors
Neutrophils
Inflammation
5-diethoxyphosphoryl-5-methyl-1-pyrroline N-oxide
1,3-dihydroxy-4,4,5,5-tetramethyl-2-(4-carboxyphenyl)tetrahydroimidazole

ASJC Scopus subject areas

  • Biochemistry

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Inhibition of superoxide generation from fMLP-stimulated leukocytes by high concentrations of nitric oxide or peroxynitrite : Characterization by electron spin resonance spectroscopy. / Lee, Masaichi Chang il; Shoji, Hirofumi; Komatsu, Tomoko; Yoshino, Fumihiko; Ohmori, Yoichi; Zweier, Jay L.

In: Redox Report, Vol. 7, No. 5, 2002, p. 271-275.

Research output: Contribution to journalArticle

Lee, Masaichi Chang il ; Shoji, Hirofumi ; Komatsu, Tomoko ; Yoshino, Fumihiko ; Ohmori, Yoichi ; Zweier, Jay L. / Inhibition of superoxide generation from fMLP-stimulated leukocytes by high concentrations of nitric oxide or peroxynitrite : Characterization by electron spin resonance spectroscopy. In: Redox Report. 2002 ; Vol. 7, No. 5. pp. 271-275.
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