Inhibition of RNA synthesis differentially affects in vitro melatonin release from the pineal organs of ayu (Plecoglossus altivelis) and rainbow trout (Oncorhynchus mykiss)

Kanta Mizusawa, Masayuki Iigo, Tomohiro Masuda, Katsumi Aida

Research output: Contribution to journalArticle

Abstract

The effects of actinomycin D (RNA synthesis inhibitor) and cycloheximide (protein synthesis inhibitor) on melatonin release from the cultured pineal organ of two teleosts with or without the circadian regulation of melatonin production (ayu Plecoglossus altivelis and rainbow trout Oncorynchus mykiss, respectively) were investigated. Actinomycin D decreased melatonin release from the pineal organ during the dark phase but there was a significant difference between the two species (22.2% for ayu and 59.1% for trout as compared with the respective control). This difference might be due to whether the circadian regulation via gene transcription of melatonin synthesis exists or not. On the other hand, cycloheximide decreased melatonin release to ∼1% in both species, indicating that the fish pineal organ requires de novo protein synthesis to maintain rhythmic melatonin release.

Original languageEnglish (US)
Pages (from-to)72-76
Number of pages5
JournalNeuroscience Letters
Volume309
Issue number1
DOIs
StatePublished - Aug 17 2001
Externally publishedYes

Fingerprint

Osmeriformes
Oncorhynchus mykiss
Melatonin
RNA
Dactinomycin
Cycloheximide
Nucleic Acid Synthesis Inhibitors
Protein Synthesis Inhibitors
Trout
In Vitro Techniques
Fishes

Keywords

  • Ayu
  • Circadian clock
  • Melatonin
  • Oncorhynchus mykiss
  • Pineal organ
  • Plecoglossus altivelis
  • Protein synthesis
  • Rainbow trout
  • RNA synthesis

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Inhibition of RNA synthesis differentially affects in vitro melatonin release from the pineal organs of ayu (Plecoglossus altivelis) and rainbow trout (Oncorhynchus mykiss). / Mizusawa, Kanta; Iigo, Masayuki; Masuda, Tomohiro; Aida, Katsumi.

In: Neuroscience Letters, Vol. 309, No. 1, 17.08.2001, p. 72-76.

Research output: Contribution to journalArticle

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abstract = "The effects of actinomycin D (RNA synthesis inhibitor) and cycloheximide (protein synthesis inhibitor) on melatonin release from the cultured pineal organ of two teleosts with or without the circadian regulation of melatonin production (ayu Plecoglossus altivelis and rainbow trout Oncorynchus mykiss, respectively) were investigated. Actinomycin D decreased melatonin release from the pineal organ during the dark phase but there was a significant difference between the two species (22.2{\%} for ayu and 59.1{\%} for trout as compared with the respective control). This difference might be due to whether the circadian regulation via gene transcription of melatonin synthesis exists or not. On the other hand, cycloheximide decreased melatonin release to ∼1{\%} in both species, indicating that the fish pineal organ requires de novo protein synthesis to maintain rhythmic melatonin release.",
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