Inhibition of protein synthesis in reticulocytes by antibiotics. V. Mechanism of action of pederine, an inhibitor of initiation and elongation

M. Jacobs-Lorena, A. Brega, C. Baglioni

Research output: Contribution to journalArticle

Abstract

The mechanism of action of pederine, an inhibitor of protein synthesis extracted from the coleopter Paederus fuscipes, has been investigated. Polysome disaggregation takes place in intact cells incubated with low concentrations (10-20 ng/ml) of pederine. At higher concentrations protein synthesis is inhibited without concomitant run off of polysomes. Polysomes incubated in the presence of pederine are inactivated irreversibly. This has suggested that pederine binds to ribosomes. Puromycin releases only 50 % of the growing polypeptide chains in the presence of pederine. Moreover, pederine inhibits the formation of N-formyl-methionylpuromycin, which occurs when the reticulocyte cell free system is incubated with N-formyl-methionyl-tRNA in the presence of puromycin. These results have suggested that pederine may interfere with the translocation of both the initiator tRNA and peptidyl-tRNA from the aminoacyl to the peptidyl site. In addition, other ribosomal functions are probably affected by this drug. Polysome disaggregation caused by pederine results most likely from an imbalance between the rate of initiation and that of elongation. At low pederine concentrations initiation seems to be preferentially inhibited. After incubation with high levels of pederine polysomes are remarkably homogeneous in sedimentation behaviour.

Original languageEnglish (US)
Pages (from-to)263-272
Number of pages10
JournalBBA Section Nucleic Acids And Protein Synthesis
Volume240
Issue number2
DOIs
StatePublished - Jun 30 1971
Externally publishedYes

ASJC Scopus subject areas

  • Medicine(all)

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