Inhibition of protein synthesis by aminoglycoside-arginine conjugates

Marjolaine Carriere, Veerappan Vijayabaskar, Drew Applefield, Isabelle Harvey, Philippe Garneau, Jon Lorsch, Aviva Lapidot, Jerry Pelletier

Research output: Contribution to journalArticlepeer-review

Abstract

Inhibition of translation by small molecule ligands has proven to be a useful tool for understanding this complex cellular mechanism, as well as providing drugs of significant medical importance. Many small molecule ligands inhibit translation by binding to RNA or RNA/protein components of the ribosomal subunits and usurping their function. A class of peptidomimetics [aminoglycoside-arginine conjugates (AAC)] has recently been designed to inhibit HIV TAR/tat interaction and in experiments aimed at assessing the inhibitory effects of AACs on TAR-containing transcripts, we found that AACs are general inhibitors of translation. Experiments reported herein aim at characterizing these novel properties of AACs. We find that AACs are inhibitors of eukaryotic and prokaryotic translation and exert their effects by blocking peptide chain elongation. Structure/activity relationship studies suggest that inhibition of translation by AACs is directly related to the number of arginine groups present on the aminoglycoside backbone and to the nature of the core aminoglycoside. AACs are therefore attractive tools for understanding and probing ribosome function.

Original languageEnglish (US)
Pages (from-to)1267-1279
Number of pages13
JournalRNA
Volume8
Issue number10
DOIs
StatePublished - Oct 1 2002

Keywords

  • Aminoglycoside-arginine conjugates
  • Peptidyl transferase inhibitor
  • Ribosome
  • Translation inhibition

ASJC Scopus subject areas

  • Molecular Biology

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