Inhibition of proliferating lens epithelium with antitransferrin receptor immunotoxin

Kenneth M. Goins, Julio R. Ortiz, Sam F.A. Fulcher, James T. Handa, Glenn J. Jaffe, Gary N. Foulks, L. Michael Cobo

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

We investigated the effect of an antitransferrin receptor immunotoxin (454A12-rRA) on proliferating human and baboon lens epithelium in vitro. Human and baboon lens epithelial cells grown in modified TC-199 medium at 35 degrees Celsius in 7% C02 were seeded in 24 well plates at a density of 17,500 cells/ml to 40,000 cells/ml. The cells were exposed to various concentrations of 454A12-rRA for seven days. The sensitivity of proliferating human lens epithelium to 454A12-rRA was dependent on the dose, with a 60% to 70% reduction in cell counts at immunotoxin concentrations of 100 ng/ml and above. The immunotoxin had no significant effect on baboon lens epithelium in vitro, which suggests that it is specific for human tissue. By preventing the proliferation of human lens epithelial cells, immunotoxin 454A12-rRA may be useful in the management of posterior capsule opacification after planned extracapsular cataract surgery.

Original languageEnglish (US)
Pages (from-to)513-516
Number of pages4
JournalJournal of cataract and refractive surgery
Volume20
Issue number5
DOIs
StatePublished - 1994
Externally publishedYes

Keywords

  • antitransferrin receptor
  • immunotoxin
  • inhibition
  • lens epithelium
  • posterior capsule opacification

ASJC Scopus subject areas

  • Surgery
  • Ophthalmology
  • Sensory Systems

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