Inhibition of proliferating lens epithelium with antitransferrin receptor immunotoxin

K. M. Goins, J. R. Ortiz, S. F A Fulcher, James Handa, G. J. Jaffe, G. N. Foulks, L. M. Cobo

Research output: Contribution to journalArticle

Abstract

We investigated the effect of an antitransferrin receptor immunotoxin (454A12-rRA) on proliferating human and baboon lens epithelium in vitro. Human and baboon lens epithelial cells grown in modified TC-199 medium at 35 degrees Celsius in 7% CO2 were seeded in 24 well plates at a density of 17,500 cells/ml to 40,000 cells/ml. The cells were exposed to various concentrations of 454A12-rRA for seven days. The sensitivity of proliferating human lens epithelium to 454A12-rRA was dependent on the dose, with a 60% to 70% reduction in cell counts at immunotoxin concentrations of 100 ng/ml and above. The immunotoxin had no significant effect on baboon lens epithelium in vitro, which suggests that it is specific for human tissue. By preventing the proliferation of human lens epithelial cells, immunotoxin 454A12-rRA may be useful in the management of posterior capsule opacification after planned extracapsular cataract surgery.

Original languageEnglish (US)
Pages (from-to)513-516
Number of pages4
JournalJournal of Cataract and Refractive Surgery
Volume20
Issue number5
StatePublished - 1994
Externally publishedYes

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Immunotoxins
Lenses
Epithelium
Papio
Epithelial Cells
Capsule Opacification
Cataract
Cell Count

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Goins, K. M., Ortiz, J. R., Fulcher, S. F. A., Handa, J., Jaffe, G. J., Foulks, G. N., & Cobo, L. M. (1994). Inhibition of proliferating lens epithelium with antitransferrin receptor immunotoxin. Journal of Cataract and Refractive Surgery, 20(5), 513-516.

Inhibition of proliferating lens epithelium with antitransferrin receptor immunotoxin. / Goins, K. M.; Ortiz, J. R.; Fulcher, S. F A; Handa, James; Jaffe, G. J.; Foulks, G. N.; Cobo, L. M.

In: Journal of Cataract and Refractive Surgery, Vol. 20, No. 5, 1994, p. 513-516.

Research output: Contribution to journalArticle

Goins, KM, Ortiz, JR, Fulcher, SFA, Handa, J, Jaffe, GJ, Foulks, GN & Cobo, LM 1994, 'Inhibition of proliferating lens epithelium with antitransferrin receptor immunotoxin', Journal of Cataract and Refractive Surgery, vol. 20, no. 5, pp. 513-516.
Goins, K. M. ; Ortiz, J. R. ; Fulcher, S. F A ; Handa, James ; Jaffe, G. J. ; Foulks, G. N. ; Cobo, L. M. / Inhibition of proliferating lens epithelium with antitransferrin receptor immunotoxin. In: Journal of Cataract and Refractive Surgery. 1994 ; Vol. 20, No. 5. pp. 513-516.
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