Inhibition of plasminogen activator secretion by cyclic AMP in a macrophage-like cell line

N. Rosen, J. Schneck, B. R. Bloom, O. M. Rosen

Research output: Contribution to journalArticlepeer-review

Abstract

The continuous cell line, J774.2, exhibits many macrophage-like functions such as latex and Fc-mediated phagocytosis, antibody mediated phagocytosis, antibody mediated cytotoxicity, chemotaxis, and lysozyme secretion. Cyclic AMP stimulates Fc-mediated phagocytosis and inhibits the growth of J774.2. To further evaluate the relationship between cyclic AMP and the specialized functions exhibited by these cells, variants deficient in phagocytosis, adenylate cyclase and cyclic AMP-dependent protein kinase were derived. The authors have now shown that J774.2 also secretes plasminogen activator and that this secretion is rapidly and specifically inhibited by 8-bromoadenosine 3':5'-cyclic monophosphoric acid (8 Br-cAMP) or cholera toxin under conditions where lysozyme secretion is unaltered. Utilizing protein kinase-deficient variants, the ability of cyclic AMP to inhibit plasminogen activator secretion was shown to be mediated by a cyclic AMP-dependent protein kinase. The authors conclude that cyclic AMP has diametrically opposing effects on two macrophage-like functions: Fc-mediated phagocytosis and plasminogen activator secretion.

Original languageEnglish (US)
Pages (from-to)345-358
Number of pages14
JournalJournal of Cyclic Nucleotide Research
Volume4
Issue number5
StatePublished - Dec 1 1978
Externally publishedYes

ASJC Scopus subject areas

  • Medicine(all)

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