Inhibition of phorbol ester-induced cellular adhesion by competitive binding of NF-κB in vivo

Stephen L. Eck, Neil D. Perkins, Dianne P. Carr, Gary J. Nabel

Research output: Contribution to journalArticle

Abstract

Adhesive interactions between cells are essential for the organization and function of differentiated tissues and organs and are mediated by inducible cell surface glycoproteins. In normal tissues, cell adhesion molecules contribute to immune regulation, inflammation, and embryogenesis. Additionally, they play an important role in a variety of pathogenic processes. Cell adhesion molecule expression can be induced by stimuli known to activate NF-κB, a ubiquitous transcription factor found in a variety of cell types. To investigate the role of NF-κB in cell adhesion molecule expression, we treated HL-60 cells with a double-stranded oligonucleotide which specifically inhibits NF-κB-mediated transcription. This treatment resulted in the inhibition of phorbol 12-myristate 13-acetate (PMA)-induced cellular adhesion, morphological changes, and the expression of leukocyte integrin CD11b. In a similar fashion, expression of intercellular adhesion molecule 1 on human endothelial cells induced by PMA was specifically inhibited by the NF-κB antagonist. We suggest that NF-κB activation is a necessary event for the PMA-induced differentiation of HL-60 cells and the expression of certain adhesion molecules. Furthermore, the inhibition of transcription factor functions by this generally applicable mechanism can be used to define their role in cellular differentiation and function.

Original languageEnglish (US)
Pages (from-to)6530-6536
Number of pages7
JournalMolecular and Cellular Biology
Volume13
Issue number10
StatePublished - Oct 1993
Externally publishedYes

Fingerprint

Competitive Binding
Cell Adhesion Molecules
Phorbol Esters
Acetates
HL-60 Cells
Transcription Factors
Tissue Adhesions
Membrane Glycoproteins
Intercellular Adhesion Molecule-1
Integrins
Oligonucleotides
Cell Communication
Adhesives
Embryonic Development
Leukocytes
Endothelial Cells
Inflammation
phorbol-12-myristate

ASJC Scopus subject areas

  • Cell Biology
  • Genetics
  • Molecular Biology

Cite this

Eck, S. L., Perkins, N. D., Carr, D. P., & Nabel, G. J. (1993). Inhibition of phorbol ester-induced cellular adhesion by competitive binding of NF-κB in vivo. Molecular and Cellular Biology, 13(10), 6530-6536.

Inhibition of phorbol ester-induced cellular adhesion by competitive binding of NF-κB in vivo. / Eck, Stephen L.; Perkins, Neil D.; Carr, Dianne P.; Nabel, Gary J.

In: Molecular and Cellular Biology, Vol. 13, No. 10, 10.1993, p. 6530-6536.

Research output: Contribution to journalArticle

Eck, SL, Perkins, ND, Carr, DP & Nabel, GJ 1993, 'Inhibition of phorbol ester-induced cellular adhesion by competitive binding of NF-κB in vivo', Molecular and Cellular Biology, vol. 13, no. 10, pp. 6530-6536.
Eck, Stephen L. ; Perkins, Neil D. ; Carr, Dianne P. ; Nabel, Gary J. / Inhibition of phorbol ester-induced cellular adhesion by competitive binding of NF-κB in vivo. In: Molecular and Cellular Biology. 1993 ; Vol. 13, No. 10. pp. 6530-6536.
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