Inhibition of p-aminophenol metabolism: A possible mechanism of enhancement of aniline hydroxylation

J. T. Stevens, K. M. Oberholser, M. R I Soliman, T. R. Brown, F. E. Greene

Research output: Contribution to journalArticle

Abstract

The p-hydroxylation of aniline has been traditionally determined by measurement of p-aminophenol (PAP) formation. Comparison of isotopic and colorimetric procedures indicate that the actual amount of aniline metabolized exceeds the amount of PAP recovered. Data suggest that enhancement of p-hydroxylation of aniline by acetone, malaoxon and paraoxon may result from inhibition of further metabolism of PAP by the microsomal cytochrome b5-dependent desaturase system. Involvement of the desaturase system is supported by observations that: (a) metabolism of PAP was reduced by starvation and stimulated when starvation was followed by feeding a high carbohydrate diet; (b) enrichment of hepatic microsomes with detergent purified cytochrome b5 decreased the amount of aniline apparently metabolized, as measured by the amount of PAP recovered, and (c) a high correlation occurred between effects of acetone, malaoxon and paraoxon on reoxidation of cytochrome b5 and capacity of these 3 compounds to enhance aniline metabolism.

Original languageEnglish (US)
Pages (from-to)153-160
Number of pages8
JournalPharmacology
Volume21
Issue number3
StatePublished - 1980
Externally publishedYes

Fingerprint

Hydroxylation
Cytochromes b5
Paraoxon
Acetone
Starvation
Microsomes
Detergents
Carbohydrates
aniline
4-aminophenol
Diet
Liver
malaoxon

ASJC Scopus subject areas

  • Pharmacology

Cite this

Stevens, J. T., Oberholser, K. M., Soliman, M. R. I., Brown, T. R., & Greene, F. E. (1980). Inhibition of p-aminophenol metabolism: A possible mechanism of enhancement of aniline hydroxylation. Pharmacology, 21(3), 153-160.

Inhibition of p-aminophenol metabolism : A possible mechanism of enhancement of aniline hydroxylation. / Stevens, J. T.; Oberholser, K. M.; Soliman, M. R I; Brown, T. R.; Greene, F. E.

In: Pharmacology, Vol. 21, No. 3, 1980, p. 153-160.

Research output: Contribution to journalArticle

Stevens, JT, Oberholser, KM, Soliman, MRI, Brown, TR & Greene, FE 1980, 'Inhibition of p-aminophenol metabolism: A possible mechanism of enhancement of aniline hydroxylation', Pharmacology, vol. 21, no. 3, pp. 153-160.
Stevens JT, Oberholser KM, Soliman MRI, Brown TR, Greene FE. Inhibition of p-aminophenol metabolism: A possible mechanism of enhancement of aniline hydroxylation. Pharmacology. 1980;21(3):153-160.
Stevens, J. T. ; Oberholser, K. M. ; Soliman, M. R I ; Brown, T. R. ; Greene, F. E. / Inhibition of p-aminophenol metabolism : A possible mechanism of enhancement of aniline hydroxylation. In: Pharmacology. 1980 ; Vol. 21, No. 3. pp. 153-160.
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AU - Oberholser, K. M.

AU - Soliman, M. R I

AU - Brown, T. R.

AU - Greene, F. E.

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