Inhibition of nitric oxide synthase does not prevent ocular dominance plasticity in kitten visual cortex

Edward S. Ruthazer, Deda C. Gillespie, Ted M Dawson, Solomon H Snyder, Michael P. Stryker

Research output: Contribution to journalArticle

Abstract

1. The neural messenger molecule nitric oxide (NO) has been shown to be involved in several forms of plasticity including hippocampal long-term potentiation. We examined the effects of chronic intracortical infusion of inhibitors of NO synthase (NOX) activity on the plasticity of visual cortical responses following monocular lid suture during the critical period. 2. Single unit recordings (618 cells) made in both the NOS inhibitor-treated (30mM N(G)-methyl-L-arginine (L-NMMA), or 22 or 2 mM nitro-L-arginine (L-NOArg)) and saline-treated control hemispheres of barbiturate-anaesthetized, critical-period kittens (n = 8) revealed a profound shift in favour of the non-deprived eye. Shifts were of similar magnitude in hemispheres in which NOS was inhibited and in saline control hemispheres. 3. Subsequent analysis of NOX activity in the same cortical tissue in which recordings had been made showed a pronounced decrease in NOX activity in inhibitor-treated hemispheres. In the region in which all the single unit recordings mere made (<3 mm from the infusion cannula), 22 mM L-NOArg resulted in a reduction of NOX activity to 5.55 ± 5.33% of control hemisphere NOX activity levels. L-NOArg (2 mar) and L-NMMA (30 mM) also produced clear, but smaller, inhibition of NOX activity. 4. These findings demonstrate that NOS activity is not essential for ocular dominance plasticity in visual cortex.

Original languageEnglish (US)
Pages (from-to)519-527
Number of pages9
JournalJournal of Physiology
Volume494
Issue number2
StatePublished - Jul 15 1996

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ocular Dominance
omega-N-Methylarginine
Visual Cortex
Nitric Oxide Synthase
Arginine
Mars
Long-Term Potentiation
Sutures
Nitric Oxide
Cannula
barbituric acid

ASJC Scopus subject areas

  • Physiology

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Inhibition of nitric oxide synthase does not prevent ocular dominance plasticity in kitten visual cortex. / Ruthazer, Edward S.; Gillespie, Deda C.; Dawson, Ted M; Snyder, Solomon H; Stryker, Michael P.

In: Journal of Physiology, Vol. 494, No. 2, 15.07.1996, p. 519-527.

Research output: Contribution to journalArticle

Ruthazer, Edward S. ; Gillespie, Deda C. ; Dawson, Ted M ; Snyder, Solomon H ; Stryker, Michael P. / Inhibition of nitric oxide synthase does not prevent ocular dominance plasticity in kitten visual cortex. In: Journal of Physiology. 1996 ; Vol. 494, No. 2. pp. 519-527.
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