Abstract
The effect of eugenol on lipoxygenase-catalyzed lipid peroxidation was studied in aqueous solution and in microsomal lipid liposomes. Eugenol did not inhibit lipoxygenase-catalyzed linoleic acid hydroperoxide formation in aqueous medium as assessed by oxygen uptake and linoleic acid hydroperoxide formation. However, using the same criteria and formation of thiobarbituric acid-reactive substances, we found that eugenol inhibited lipid oxygenation when the reaction was carried out in linoleic acid-containing liposomes. These results suggest that the peroxyl and carbon-centered radical intermediates generated by lipoxygenase in a membrane environment are readily accessible to eugenol for quenching and concomitant inhibition of lipid peroxidation.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 123-129 |
| Number of pages | 7 |
| Journal | Journal of Clinical Biochemistry and Nutrition |
| Volume | 21 |
| Issue number | 2 |
| DOIs | |
| State | Published - Sep 1996 |
| Externally published | Yes |
Keywords
- eugenol
- lipid peroxidation
- lipoxygenase
- microsomal lipid liposomes
ASJC Scopus subject areas
- Medicine (miscellaneous)
- Nutrition and Dietetics
- Clinical Biochemistry