Inhibition of in vivo tumorigenicity and invasiveness of a human glioblastoma cell line transfected with antisense uPAR vectors

Yoshinori Go, Shravan K. Chintala, Sanjeeva Mohanam, Ziya Gokaslan, Boyapati Venkaiah, Rolf Bjerkvig, Kazunari Oka, Garth L. Nicolson, Raymond Sawaya, Jasti S. Rao

Research output: Contribution to journalArticle

Abstract

Our previous studies showed that glioblastomas express increased urokinase-type plasminogen activator receptors (uPARs) in comparison to low-grade gliomas (Yamamoto et al., Cancer Res.. 54, 5016-5020, 1994). To explore whether donwnregulation of uPAR inhibits tumor formation and invasiveness, a human glioblastoma cell line was transfected with a cDNA construct corresponding to 300 bp of the human uPAR's 5' end in an antisense orientation, resulting in a reduced number of uPA receptors. Co-culture studies with tumor spheroids and fetal rat brain aggregates showed that antisense SNB19-AS1 cells expressing reduced uPAR failed to invade fetal rat brain aggregates. Intracerebral injection of SNB19-AS1 stable transfectants failed to form tumors and were negative for uPAR expression in nude mice. Thus uPAR appears in this model to be essential for tumorigenicity and invasion of glioblastomas in vivo.

Original languageEnglish (US)
Pages (from-to)440-446
Number of pages7
JournalClinical and Experimental Metastasis
Volume15
Issue number4
DOIs
StatePublished - Jul 22 1997

Keywords

  • Activators
  • Antisense
  • Brain
  • Glioblastoma
  • Plasminogen
  • Tumor
  • lacZ
  • uPAR

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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  • Cite this

    Go, Y., Chintala, S. K., Mohanam, S., Gokaslan, Z., Venkaiah, B., Bjerkvig, R., Oka, K., Nicolson, G. L., Sawaya, R., & Rao, J. S. (1997). Inhibition of in vivo tumorigenicity and invasiveness of a human glioblastoma cell line transfected with antisense uPAR vectors. Clinical and Experimental Metastasis, 15(4), 440-446. https://doi.org/10.1023/A:1018410523635