Inhibition of DNA binding protein binding to the core promoter of hepatitis B virus by triplex-formation

Ding Xie Liu, Chang Cai Wang

Research output: Contribution to journalArticlepeer-review

Abstract

The triplex formation between a 21nt oligodeoxyribonucleotide G3TG2TGT2G5TG2TGT (CP1) and core promoter (Cp) fragment of hepatitis B virus (HBV) had a good specificity and stability, which was demonstrated by electrophoretic mobility shift analysis and DNase I footprinting experiment. Gel retardation assay showed CP1 could inhibit a specific cellular factor binding to Cp fragment in rat liver nuclear extracts. No inhibition of factor binding was observed by oligodeoxyribonucleotide CP3 (TGTG2TG5T2GTG2TG3), which could not form triplex with Cp fragment. These results indicate that specific repression of gene transcription of HBV DNA may be possible by triplex-formation.

Original languageEnglish (US)
Pages (from-to)265-266
Number of pages2
JournalProgress in Biochemistry and Biophysics
Volume25
Issue number3
StatePublished - 1998
Externally publishedYes

Keywords

  • Antigene strategy
  • DNA binding protein
  • Hepatitis B virus
  • Oligodeoxyribonucleotide
  • Triplex DNA

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics

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